Krantz-Rülcker C, Frändberg E, Schnürer J
Department of Water and Environmental Studies, Linköping University, Sweden.
Biometals. 1995 Jan;8(1):12-8. doi: 10.1007/BF00156152.
The capacity of chitin (from crab shells) and of fungal cell walls from Trichoderma harzianum to accumulate zinc, cadmium and mercury was studied as well as the effects of adsorbed metals on the enzymatic hydrolysis by Novozym 234 of the two substrates. The total adsorbing capacity with respect to these metals was estimated to be at least 10 mmol kg-1 chitin (dry weight) and 50 mmol kg-1 fungal cell walls (dry weight), respectively, at pH 6.1. Enzymatic digestion of fungal cell walls preloaded with mercury and cadmium was significantly reduced, while zinc did not cause any significant inhibition. The effect of metal complexation by chitin on the enzymatic digestion was not as pronounced as for fungal cell walls. This could reflect the fact that chitin sorbed a lower total amount of metals. The inhibitory effect of metals on the enzymatic hydrolysis was caused by the association of the metals with the two substrates and not by the presence of free metals in solution.
研究了几丁质（来自蟹壳）和哈茨木霉真菌细胞壁积累锌、镉和汞的能力，以及吸附的金属对这两种底物经诺维信234进行酶促水解的影响。在pH 6.1时，相对于这些金属的总吸附容量估计分别为至少10 mmol·kg⁻¹几丁质（干重）和50 mmol·kg⁻¹真菌细胞壁（干重）。预先负载汞和镉的真菌细胞壁的酶促消化显著降低，而锌未引起任何显著抑制。几丁质对金属的络合作用对酶促消化的影响不如真菌细胞壁明显。这可能反映了几丁质吸附的金属总量较低这一事实。金属对酶促水解的抑制作用是由金属与两种底物的结合引起的，而不是溶液中游离金属的存在导致的。
