Thomas K R, Davis B, Mills J
Microbios. 1979;25(100):111-23.
The effects of beta-glucuronidase and chitinase have been tested on the hydrolysis of the cell walls of the economically important fungi, Aspergillus niger and Aspergillus fumigatus. The extent of wall hydrolysis was measured by assaying for total reducing sugars, N-acetyl sugars and protoplast production. Maximum reducing sugar release was attained after 40 min incubation, both with beta-glucuronidase supplemented with chitinase and beta-glucuronidase alone, whereas N-acetyl sugar release reached a maximum at 80 min incubation. beta-Glucuronidase was effective in releasing protoplasts from both species of Aspergillus. This release was enhanced by adding chitinase to the incubation medium at 0 and 20 min, but with addition at 60, 80 and 100 min increase in protoplast yield was much reduced. The results of re-incubation experiments with chitinase suggest that this enzyme may in some way be inhibited during the later stages of incubation. Pronase used in combination with beta-glucuronidase slightly enhanced protoplast release.
已测试β-葡萄糖醛酸酶和几丁质酶对经济上重要的真菌黑曲霉和烟曲霉细胞壁水解的影响。通过测定总还原糖、N-乙酰糖和原生质体产量来衡量细胞壁水解的程度。在添加几丁质酶的β-葡萄糖醛酸酶和单独的β-葡萄糖醛酸酶孵育40分钟后，还原糖释放量均达到最大值，而N-乙酰糖在孵育80分钟时释放量达到最大值。β-葡萄糖醛酸酶可有效从两种曲霉中释放原生质体。在0和20分钟时向孵育培养基中添加几丁质酶可增强原生质体的释放，但在60、80和100分钟时添加，原生质体产量的增加则大大减少。几丁质酶再孵育实验的结果表明，该酶在孵育后期可能会以某种方式受到抑制。与β-葡萄糖醛酸酶联合使用的链霉蛋白酶可略微增强原生质体的释放。
