基于金纳米颗粒的侧向流条的研制及其在鸡肉组织中二硝托胺残留检测中的应用。
Development of a gold nanoparticle-based lateral-flow strip for the detection of dinitolmide in chicken tissue.
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
出版信息
Anal Methods. 2020 Jul 2;12(25):3210-3217. doi: 10.1039/d0ay00885k.
Dinitolmide is a nitro amide coccidiostat used in poultry feed, and is a potential threat to the environment and human health. In this study, a monoclonal antibody (mAb) against dinitolmide was prepared and an immunochromatographic assay (ICA) was developed to detect residual dinitolmide in chicken tissue. The results show that the mAb exhibited high sensitivity, with a limit of detection as low as 9.01 ng mL-1. A cross reactivity test revealed that the mAb also had good specificity for dinitolmide. This ICA method showed a visible limit of detection of 2.5 μg kg-1, and a cut-off value of 25 μg kg-1 for testing dinitolmide in chicken sample extract by using the naked eye. Importantly, these observations using our ICA, were comparable to other methods of detection such as liquid chromatography, tandem mass spectrometry and indirect competitive enzyme-linked immunosorbent assays (IC-ELISA). These data suggest that our ICA method is a reliable, portable, and high-throughput method for the detection of dinitolmide residues in chicken tissue.
地克珠利是一种用于家禽饲料的硝酰胺类抗球虫药，对环境和人类健康构成潜在威胁。本研究制备了一种针对地克珠利的单克隆抗体（mAb），并开发了一种免疫层析分析（ICA）方法来检测鸡肉组织中的地克珠利残留。结果表明，该 mAb 具有较高的灵敏度，检测限低至 9.01ng/mL。交叉反应试验表明，该 mAb 对地克珠利也具有良好的特异性。该 ICA 方法对鸡肉样品提取物中地克珠利的肉眼可见检测限为 2.5μg/kg，截止值为 25μg/kg。重要的是，使用我们的 ICA 观察到的这些结果与其他检测方法（如液相色谱、串联质谱和间接竞争酶联免疫吸附测定（IC-ELISA））相当。这些数据表明，我们的 ICA 方法是一种可靠、便携且高通量的检测鸡肉组织中地克珠利残留的方法。
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