基于金纳米粒子的侧向流条快速灵敏检测马铃薯和南瓜样品中的氯氨哒嗪。
Rapid and sensitive detection of clomazone in potato and pumpkin samples using a gold nanoparticle-based lateral-flow strip.
机构信息
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
出版信息
Food Chem. 2022 May 1;375:131888. doi: 10.1016/j.foodchem.2021.131888. Epub 2021 Dec 18.
In this study, an ultrasensitive monoclonal antibody (mAb) was produced and used to develop a gold nanoparticle-based lateral flow immunochromatographic (ICA) strip for screening of clomazone (CLO) in potato and pumpkin samples. With assayed by indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method, the mAb belonging of IgG2 subclass showed a half-maximal inhibitory concentration (IC50) of 3.47 ng/mL and a linear range of detection of 0.43-28.09 ng/mL. A cross-reactivity test revealed that the mAb had good specificity for CLO. The strip assay had a visual limit of detection (LOD) of 5 µg/kg and a cut-off value of 50 µg/kg for CLO pumpkin samples (potato samples was 100 µg/kg) when evaluated with the naked eye. The results were consistent with ic-ELISA and high performance liquid chromatography tandem mass spectrometry (HPLC-MS). Thus, this ICA strip assay represents a potentially tool for on-site and rapid initial detection of CLO in potato and pumpkin samples.
本研究制备了一种超灵敏单克隆抗体（mAb），并将其用于开发基于金纳米颗粒的侧向流动免疫层析（ICA）条，用于筛选马铃薯和南瓜样品中的氯甲唑（CLO）。通过间接竞争酶联免疫吸附试验（ic-ELISA）方法测定，该 mAb 属于 IgG2 亚类，其半抑制浓度（IC50）为 3.47ng/mL，检测线性范围为 0.43-28.09ng/mL。交叉反应试验表明，该 mAb 对 CLO 具有良好的特异性。该条带检测法的目视检测限（LOD）为 5µg/kg，当用肉眼评估时，南瓜样品（马铃薯样品为 100µg/kg）的截断值为 50µg/kg。结果与 ic-ELISA 和高效液相色谱串联质谱（HPLC-MS）一致。因此，该 ICA 条带检测法代表了一种用于现场和快速初步检测马铃薯和南瓜样品中 CLO 的潜在工具。
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