基于金的免疫层析检测条用于检测食品中的喹草酮。

Gold-based immunochromatographic assay strip for the detection of quinclorac in foods.

机构信息

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

出版信息

Analyst. 2021 Nov 8;146(22):6831-6839. doi: 10.1039/d1an01748a.

DOI:10.1039/d1an01748a

PMID:34723310

Abstract

In this study, a highly specific and sensitive monoclonal antibody (mAb) against quinclorac (Qui) was prepared. Based on the selected mAb, 2G3, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic strip assay were established for the detection of Qui in actual samples. The 50%-inhibitory concentration of mAb 2G3 against Qui was 48.763 ng mL. No cross-reaction with other quinolines indicated that mAb 2G3 had high specificity. The recovery of the established ic-ELISA method was in the range of 85.6% to 98.9%. The cut-off value of Qui in cucumber and tomato by immunochromatographic strip was 200 ng g. The analysis results of ic-ELISA and immunochromatographic strip assay were consistent with the results of LC-MS/MS, which further proved that the established ic-ELISA and immunochromatographic strip assay could provide valuable tools for the rapid detection of Qui residues in cucumber and tomato samples.

摘要

本研究制备了一种针对喹禾灵（Qui）的高特异性和高灵敏度单克隆抗体（mAb）。基于所选择的 mAb 2G3，建立了间接竞争酶联免疫吸附测定（ic-ELISA）和免疫层析条检测法，用于检测实际样品中的 Qui。mAb 2G3 对 Qui 的 50%抑制浓度为 48.763 ng mL。与其他喹啉类化合物无交叉反应表明 mAb 2G3 具有高特异性。建立的 ic-ELISA 方法的回收率在 85.6%至 98.9%之间。免疫层析条法在黄瓜和番茄中 Qui 的检测限为 200 ng g。ic-ELISA 和免疫层析条检测结果与 LC-MS/MS 结果一致，进一步证明建立的 ic-ELISA 和免疫层析条检测法可用于快速检测黄瓜和番茄样品中的 Qui 残留。