Department of Physical Education, Gyeongsang National University, Jinju, Republic of Korea; Department of Parasitology and Tropical Medicine, Pusan National University School of Medicine, Yangsan, Republic of Korea; Department of Orthopaedic Surgery, Gyeongsang National University Hospital, Jinju, Republic of Korea.
Department of Parasitology and Tropical Medicine, Pusan National University School of Medicine, Yangsan, Republic of Korea.
Exp Gerontol. 2020 Nov;141:111091. doi: 10.1016/j.exger.2020.111091. Epub 2020 Sep 12.
In the adipose tissue (AT), an increase in the M1 macrophage (M1Ø)/M2 macrophage (M2Ø) polarization ratio can be a risk factor enhancing the inflammatory response during aging, as well as increasing the risk of chronic disease, thereby reducing lifespan, or at least reducing "healthy" lifespan. The purpose of this study was to analyze and compare the AT M1Ø/M2Ø polarization ratio at the final lifespan stage in aged and control animals performing lifelong spontaneous wheel running. Based on flow cytometric analysis, the AT ratio of macrophages revealed M2Ø polarization following lifelong spontaneous exercise (LSE) regardless of age. However, for Icam1 and Tnf, the qPCR analysis showed no difference in gene expressions in young mice; Arg1 expression was higher in Young-EXE (exercising) than in Young-CON (control) mice (p < .0001). In Old-EXE, Icam1 (p < .0001) and Tnf (p < .0001) expression were lower than in Old-CON; for Arg1, gene expression in Old-EXE was higher than in Old-CON (p < .0001). LSE prevents deterioration of physical fitness owing to aging, maintaining high M2Ø polarization levels in the AT. Additionally, LSE does not downregulate Icam1 and Tnf in the AT but appears to suppress the increased M1Ø polarization ratio attributed to aging by upregulating Arg1.
在脂肪组织（AT）中，M1 巨噬细胞（M1Ø）/M2 巨噬细胞（M2Ø）极化比率的增加可能是增强衰老过程中炎症反应的风险因素，增加患慢性疾病的风险，从而缩短寿命，或者至少缩短“健康”寿命。本研究的目的是分析和比较终身自发轮跑的老年和对照动物在终末寿命阶段的 AT M1Ø/M2Ø 极化比率。基于流式细胞术分析，无论年龄如何，AT 中巨噬细胞的比率显示 M2Ø 极化后进行终身自发运动（LSE）。然而，对于 Icam1 和 Tnf，qPCR 分析显示年轻小鼠的基因表达没有差异；Arg1 表达在 Young-EXE（运动）小鼠中高于 Young-CON（对照）小鼠（p <.0001）。在 Old-EXE 中，Icam1（p <.0001）和 Tnf（p <.0001）的表达低于 Old-CON；对于 Arg1，Old-EXE 中的基因表达高于 Old-CON（p <.0001）。LSE 可防止因衰老导致的身体机能恶化，保持 AT 中高 M2Ø 极化水平。此外，LSE 不会下调 AT 中的 Icam1 和 Tnf，但似乎通过上调 Arg1 来抑制归因于衰老的 M1Ø 极化比率增加。
