Baek Kyung-Wan, Kim Ji Hyun, Yu Hak Sun, Kim Ji-Seok
Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea.
Department of Physical Education, Gyeongsang National University, Jinju 52828, Republic of Korea.
Curr Issues Mol Biol. 2024 Feb 2;46(2):1308-1317. doi: 10.3390/cimb46020083.
We performed a large-scale flow cytometric analysis to determine whether M1 macrophage (M1Ø) and M2 macrophage (M2Ø) polarization in white adipose tissue (WAT) was altered immediately after exercise. Additionally, we comprehensively investigated the effects of obesity, exercise intensity, and recovery time on macrophage polarization in WAT. A single exercise bout of various intensities (ND, non-exercise control; -LIE, low-intensity exercise; -MIE, mid-intensity exercise; -HIE, high-intensity exercise) was performed by normal mice (ND) and obese mice (HFD). To confirm differences in M1Ø/M2Ø polarization in WAT based on the recovery time after a single exercise bout, WAT was acquired at 2 h, 24 h, and 48 h after exercise (total = 168, 7 mice × 4 groups × 2 diets × 3 recovery time). The harvested WAT was immediately analyzed by flow cytometry, and macrophages were fluorescently labeled using F4/80, as well as M1Ø with CD11c and M2Øs with CD206. After a single bout of exercise, the M2Ø/M1Ø polarization ratio of WAT increases in both normal and obese mice, but differences vary depending on recovery time and intensity. Regardless of obesity, our findings showed that there could be a transient increase in M1Ø in WAT over a short recovery time (24 h) post-exercise (in ND-MIE, ND-HIE, and HFD-HIE). Furthermore, it was observed that the greater the exercise intensity in obese mice, the more effective the induction of M2Ø polarization immediately after exercise, as well as the maintenance of high M2Ø polarization, even after a prolonged recovery time.
我们进行了大规模流式细胞术分析，以确定运动后白色脂肪组织（WAT）中的M1巨噬细胞（M1Ø）和M2巨噬细胞（M2Ø）极化是否立即发生改变。此外，我们全面研究了肥胖、运动强度和恢复时间对WAT中巨噬细胞极化的影响。正常小鼠（ND）和肥胖小鼠（HFD）进行了一次不同强度的单次运动（ND，非运动对照；-LIE，低强度运动；-MIE，中等强度运动；-HIE，高强度运动）。为了确认单次运动后基于恢复时间的WAT中M1Ø/M2Ø极化差异，在运动后2小时、24小时和48小时采集WAT（共168个样本，7只小鼠×4组×2种饮食×3个恢复时间）。收获的WAT立即通过流式细胞术进行分析，巨噬细胞用F4/80进行荧光标记，M1Ø用CD11c标记，M2Ø用CD206标记。单次运动后，正常和肥胖小鼠WAT的M2Ø/M1Ø极化率均增加，但差异因恢复时间和强度而异。无论肥胖情况如何，我们的研究结果表明，运动后短时间恢复（24小时）内WAT中的M1Ø可能会短暂增加（在ND-MIE、ND-HIE和HFD-HIE中）。此外，观察到肥胖小鼠的运动强度越大，运动后立即诱导M2Ø极化的效果越明显，并且即使在长时间恢复后仍能维持高M2Ø极化。
