College of Chemistry and Food Engineering, Changsha University of Science & Technology, Changsha 410114, China.
College of Chemistry and Food Engineering, Changsha University of Science & Technology, Changsha 410114, China.
Food Chem. 2021 Feb 15;338:128039. doi: 10.1016/j.foodchem.2020.128039. Epub 2020 Sep 8.
In order to avoid the occurrence of false positives and false negatives caused by conventional enzyme-linked immunosorbent assay (ELISA), we established a novel indirect competitive MOF-linked immunosorbent assay (MOFLISA) method for the high throughput and high sensitive detection of aflatoxin B. This method replaces the natural enzyme with functional MOFs to catalyze a chromogenic system. As a result, the limit of detection (LOD) of the MOFLISA method was 0.009 ng·mL with a linear working range from 0.01 to 20 ng·mL. The developed MOFLISA method for AFB has a 20-fold improved LOD value compared with the conventional ELISA. The recoveries and relative standard deviations (RSD) ranged from 86.41 to 99.74% and 2.38-9.04%, respectively. The results demonstrate that the recovery rate and accuracy of this detection method is better than that of conventional ELISA, reducing risks offalsepositive andfalsenegativeresults.
为了避免传统酶联免疫吸附测定（ELISA）中出现假阳性和假阴性的情况，我们建立了一种新型的间接竞争金属有机框架（MOF）连接免疫吸附测定（MOFLISA）方法，用于高通量和高灵敏度地检测黄曲霉毒素 B。该方法用功能化的 MOF 替代天然酶来催化显色体系。结果，MOFLISA 方法的检测限（LOD）为 0.009ng·mL，线性工作范围为 0.01 至 20ng·mL。与传统 ELISA 相比，开发的用于 AFB 的 MOFLISA 方法的 LOD 值提高了 20 倍。回收率和相对标准偏差（RSD）分别在 86.41%至 99.74%和 2.38%至 9.04%之间。结果表明，该检测方法的回收率和准确性优于传统 ELISA，降低了假阳性和假阴性结果的风险。
