Department of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, PO Box: 27272, Sharjah, United Arab Emirates; Sharjah Institute for Medical Research, University of Sharjah, PO Box: 27272, Sharjah, United Arab Emirates.
RAKCODS, RAK Medical & Health Sciences University, PO Box: 12973, Ras Al Khaimah, United Arab Emirates.
Arch Oral Biol. 2020 Nov;119:104896. doi: 10.1016/j.archoralbio.2020.104896. Epub 2020 Sep 4.
This study investigates the post-extraction storage period of human dental pulp stem cells (hDPSCs) for stem cell banking by investigating the viability, function, mineralization, and gene expression of hDPSCs isolated from extracted teeth after 1 h, 6 h and 24 h post-tooth extraction.
hDPSCs were extracted from the pulp of impacted third molar teeth after 1 h, 6 h, and 24 h after extraction. The mesenchymal stem cell (MSCs) properties of three groups of cells were analyzed using flow cytometry. Cell morphology and proliferation were analyzed using a light microscope and an MTT assay. The viability, function, mineralization, and gene expression of hDPSCs of 1 h, 6 h, and 24 h groups were also assessed.
The delayed harvesting of hDPSCs for 1, 6 or 24 h caused a 31 % reduction in mineral nodule formation and a reduction in the gene expression of osteocalcin (OCN) and vascular endothelial growth factor-alpha (VEGFA). However, the 1, 6 or 24 h, time delay had little effect on MTT cell proliferation, cell viability or morphology. The delayed of harvesting of hDPSCs for 1, 6 or 24 h also had little effect on the expression of MSCs positive (CD44, CD106, CD90) or negative surface markers (CD45 and CD11b).
Our results suggest that a 24 h delay in harvesting hDPSCs from extracted teeth can reduce their mineralization and gene activity but does not markedly reduce survival. Quicker hDPSCs harvesting is likely to yield more useful hDPSCs for experimentation and clinical treatment.
本研究通过研究从拔牙后 1 h、6 h 和 24 h 提取的牙齿中分离的人牙髓干细胞（hDPSCs）的活力、功能、矿化和基因表达，来研究 hDPSCs 的提取后储存时间，以用于干细胞库。
从拔除后 1 h、6 h 和 24 h 的第三磨牙牙髓中提取 hDPSCs。使用流式细胞术分析三组细胞的间充质干细胞（MSCs）特性。使用相差显微镜和 MTT 测定法分析细胞形态和增殖。还评估了 1 h、6 h 和 24 h 组 hDPSCs 的活力、功能、矿化和基因表达。
hDPSCs 延迟收获 1、6 或 24 h 会导致矿化结节形成减少 31%，并降低骨钙素（OCN）和血管内皮生长因子-α（VEGFA）的基因表达。然而，1、6 或 24 h 的时间延迟对 MTT 细胞增殖、细胞活力或形态几乎没有影响。hDPSCs 延迟收获 1、6 或 24 h 也几乎不影响 MSC 阳性（CD44、CD106、CD90）或阴性表面标志物（CD45 和 CD11b）的表达。
我们的结果表明，从拔牙中提取 hDPSCs 后延迟 24 h 会降低其矿化和基因活性，但不会明显降低存活率。更快地收获 hDPSCs 可能会产生更有用的 hDPSCs 用于实验和临床治疗。
