De Visscher Justine, Vermeir Lore, Van den Vreken Natasja, Temmerman Liesbeth, De Roo Noëmi, van Hengel Jolanda, De Pauw Guy
Department of Oral Health Sciences, Ghent University, Corneel Heymanslaan 10, 9000, Ghent, Belgium.
Medical Cell Biology Research Group, Department of Human Structure and Repair, Ghent University, Corneel Heymanslaan 10, 9000, Ghent, Belgium.
Cell Tissue Bank. 2025 Jul 21;26(3):32. doi: 10.1007/s10561-025-10183-2.
The purpose of this study was to compare two methods for harvesting human dental pulp stem cells (hDPSCs) and investigate their impact on the DPSC viability and differentiation capacity. Healthy premolar teeth were collected from children aged 9 - 17 years old undergoing orthodontic treatment with two or four premolar extractions. The included premolars were randomly allocated to the apical group (removal of the dental pulp through the apical foramen after tooth extraction) or the coronal group (isolating the pulp tissue through the crown prior to tooth extraction). A total of 148 healthy premolar teeth from 46 patients were extracted, including 74 pulp tissues for the apical group and 74 for the coronal group. Due to bacterial and fungal infections, 37 (18 apical and 19 coronal) DPSC lines were obtained. Flow cytometric analysis and MTT assays showed no statistical differences in cell viability between the two methods. Odontogenic differentiation, assessed through immunocytochemical staining and Alizarin Red staining, also showed no visual or statistical differences between the apical and coronal approaches. In conclusion, the biological characteristics of hDPSCs harvested through the crown were equal to apically prelevated hDPSCs.
本研究的目的是比较两种获取人牙髓干细胞（hDPSCs）的方法，并研究它们对牙髓干细胞活力和分化能力的影响。从9至17岁接受正畸治疗且需拔除两颗或四颗前磨牙的儿童中收集健康的前磨牙。纳入的前磨牙被随机分配至根尖组（拔牙后通过根尖孔去除牙髓）或冠部组（拔牙前通过牙冠分离牙髓组织）。共从46例患者中拔除了148颗健康前磨牙，其中根尖组74个牙髓组织，冠部组74个。由于细菌和真菌感染，共获得37条（根尖组18条，冠部组19条）牙髓干细胞系。流式细胞术分析和MTT检测显示，两种方法在细胞活力方面无统计学差异。通过免疫细胞化学染色和茜素红染色评估的成牙分化，在根尖和冠部取材方法之间也未显示出视觉或统计学差异。总之，通过牙冠获取的人牙髓干细胞的生物学特性与通过根尖获取的牙髓干细胞相当。
