Department of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
J Oral Pathol Med. 2011 Nov;40(10):793-800. doi: 10.1111/j.1600-0714.2011.01040.x. Epub 2011 Apr 23.
Successful isolation of human dental pulp stem cells (hDPSCs) has been documented at least 120h after tooth extraction. Viable hDPSCs have been isolated chiefly from cryopreserved healthy molar teeth and their undigested dental pulp tissue. Isolation of hDPSCs from diseased but vital teeth after cryopreservation has not been reported. This study aimed to isolate hDPSCs from cryopreserved diseased but vital teeth of various tooth types.
Fifty tooth samples were divided into group A (n = 20) - freshly derived dental pulp tissues, group B (n = 20) - liquid nitrogen (liq N(2) )-stored dental pulp tissues and group C (n = 10) - liq N(2) -stored intact teeth.
The success rate for hDPSCs isolation was 100% for groups A and B and only 20% for group C. hDPSCs from all groups demonstrated self-renewal properties and similar multipotent potential characteristics of adipogenic, chondrogenic and osteogenic differentiation. In addition, hDPSCs showed high expression of bone-marrow mesenchymal stem-cell markers (CD29, CD90 and CD105) and very low expression of specific hematopoietic cells markers (CD14, CD34 and CD45).
Our results indicate that hDPSCs isolated from diseased but vital teeth of various tooth types can be stored in liq N(2) for future usage.
从拔牙后至少 120 小时起，已经成功分离出人类牙髓干细胞（hDPSCs）。主要从冷冻保存的健康磨牙及其未消化的牙髓组织中分离出有活力的 hDPSCs。尚未报道从冷冻保存的患病但有活力的牙齿中分离 hDPSCs。本研究旨在从各种类型的冷冻保存的患病但有活力的牙齿中分离 hDPSCs。
50 个牙样本分为 A 组（n=20）-新鲜牙髓组织、B 组（n=20）-液氮（liq N(2)）储存的牙髓组织和 C 组（n=10）-液氮（liq N(2)）储存的完整牙齿。
hDPSCs 分离的成功率为 A 组和 B 组 100%，C 组仅为 20%。所有组的 hDPSCs 均表现出自体更新特性和相似的多能潜能特性，包括脂肪生成、软骨生成和成骨分化。此外，hDPSCs 表现出高表达骨髓间充质干细胞标志物（CD29、CD90 和 CD105）和极低表达特定造血细胞标志物（CD14、CD34 和 CD45）。
我们的结果表明，从各种类型的患病但有活力的牙齿中分离出的 hDPSCs 可以储存在 liq N(2) 中以备将来使用。
