Laboratório de Diferenciação Muscular e Citoesqueleto, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Instituto Nacional de Câncer, Rio de Janeiro, RJ, Brazil.
Tissue Cell. 2020 Dec;67:101436. doi: 10.1016/j.tice.2020.101436. Epub 2020 Sep 1.
CAPN3 is a muscle-specific and an intrinsically disordered protein. Thus, as a scaffolding protein CAPN3 could play a role during early stages of myogenesis. To test this hypothesis, we studied the distribution and function of CAPN3 during myogenesis using embryonic chick muscle cells grown in vitro. Super-resolution microscopy showed CAPN3 distribution in (i) amorphous patches in myoblasts, (ii) a region near the nuclei of myotubes; (iii) adhesion plaques in myotubes, (iv) stress fiber-like structures in myotubes, and (v) filaments in fibroblasts. Downregulation of CAPN3 induced a decrease in the number of muscle cells and in the size of myotubes formed. These data show a diverse intracellular distribution of CAPN3, compatible with a scaffolding protein, and suggest a multitude of different interactions of CAPN3 with other partners during muscle formation.
CAPN3 是一种肌肉特异性的无序蛋白。因此，作为一种支架蛋白，CAPN3 可能在肌发生的早期阶段发挥作用。为了验证这一假说，我们使用体外培养的鸡胚肌肉细胞研究了 CAPN3 在肌发生过程中的分布和功能。超分辨率显微镜显示 CAPN3 在（i）成肌细胞中的无定形斑，（ii）肌管细胞核附近区域，（iii）肌管黏附斑，（iv）肌管应力纤维样结构，和（v）成纤维细胞中的细丝中的分布。CAPN3 的下调导致肌肉细胞数量减少和形成的肌管变小。这些数据显示 CAPN3 具有多样化的细胞内分布，与支架蛋白一致，并表明在肌肉形成过程中 CAPN3 与其他伴侣有多种不同的相互作用。
