Baird M A, Munn S R, Heslop B F
Department of Surgery, University of Otago Medical School, Dunedin, New Zealand.
Transplantation. 1988 Jul;46(1):105-8. doi: 10.1097/00007890-198807000-00019.
Pretransplant transfusions of heat-treated spleen and lymph node cells were shown to prolong the survival of DA strain heart grafts in 3 allogeneic host strains: BS, HS, and AS2. To examine whether MHC incompatibility was necessary for immunosuppression mediated by heat-treated cells, AS strain skin-graft recipients were pretreated with fresh or heated inocula from either MHC compatible or incompatible congenic donor strains, AS2.1L(AS) and AS.1F(AS2) prior to transplanting donor strain skin. Prolonged survival was observed only in the MHC-incompatible strain combination, and in this MHC-incompatible strain combination, and in this instance heated cells were conspicuously more immunosuppressive than fresh cells. To determine the effect of intra-MHC differences between donor and host on graft survival, cells from a recombinant donor strain (r22), which shared class II antigens with the graft donor strain and class I antigens with the host, were transfused prior to heart transplantation. Neither fresh nor heated r22 cells prolonged graft survival. Our data accord with the suggestion that in the absence of MHC-compatible antigen-presenting cells, foreign class I antigen is immunosuppressive.
经热处理的脾脏和淋巴结细胞的移植前输血已被证明可延长DA品系心脏移植物在3种异基因宿主品系（BS、HS和AS2）中的存活时间。为了研究MHC不相容性对于由热处理细胞介导的免疫抑制是否必要，在移植供体品系皮肤之前，用来自MHC相容或不相容的同基因供体品系AS2.1L(AS)和AS.1F(AS2)的新鲜或加热接种物对AS品系皮肤移植受体进行预处理。仅在MHC不相容的品系组合中观察到存活时间延长，并且在这种MHC不相容的品系组合中，在这种情况下，加热的细胞比新鲜细胞具有明显更强的免疫抑制作用。为了确定供体和宿主之间MHC内差异对移植物存活的影响，在心脏移植前输注来自重组供体品系（r22）的细胞，该品系与移植物供体品系共享II类抗原，与宿主共享I类抗原。新鲜或加热的r22细胞均未延长移植物存活时间。我们的数据与以下观点一致：在没有MHC相容的抗原呈递细胞的情况下，外来的I类抗原具有免疫抑制作用。
