Department of General and Medical Biochemistry, Faculty of Biology, University of Gdańsk, 80-308 Gdańsk, Poland.
University Centre of Veterinary Medicine, University of Agriculture in Krakow, 30-059 Krakow, Poland.
Int J Mol Sci. 2020 Sep 11;21(18):6645. doi: 10.3390/ijms21186645.
Bacterial ClpB is an ATP-dependent disaggregase that belongs to the Hsp100/Clp subfamily of the AAA+ ATPases and cooperates with the DnaK chaperone system in the reactivation of aggregated proteins, as well as promotes bacterial survival under adverse environmental conditions, including thermal and oxidative stresses. In addition, extensive evidence indicates that ClpB supports the virulence of numerous bacteria, including pathogenic spirochaete responsible for leptospirosis in animals and humans. However, the specific function of ClpB in leptospiral virulence still remains to be fully elucidated. Interestingly, ClpB was predicted as one of the hub proteins interacting with human proteins, and pathogen-host protein interactions are fundamental for successful invasion of the host immune system by bacteria. The aim of this review is to discuss the most important aspects of ClpB's function in , including contribution of ClpB to leptospiral virulence and pathogenesis of leptospirosis, a zoonotic disease with a significant impact on public health worldwide.
细菌 ClpB 是一种依赖于 ATP 的解聚酶，属于 Hsp100/Clp 家族的 AAA+ATP 酶，与 DnaK 伴侣系统合作，在重新激活聚集蛋白方面发挥作用，并促进细菌在不利的环境条件下存活，包括热应激和氧化应激。此外，大量证据表明 ClpB 支持许多细菌的毒力，包括引起动物和人类钩端螺旋体病的致病性螺旋体。然而，ClpB 在钩端螺旋体毒力中的具体作用仍有待充分阐明。有趣的是，ClpB 被预测为与人类蛋白相互作用的 个枢纽蛋白之一，病原体-宿主蛋白相互作用是细菌成功入侵宿主免疫系统的基础。本文旨在讨论 ClpB 在钩端螺旋体中的功能的重要方面，包括 ClpB 对钩端螺旋体毒力和钩端螺旋体病发病机制的贡献，钩端螺旋体病是一种对全球公共卫生有重大影响的人畜共患疾病。
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