School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, China.
National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.
Nutrients. 2020 Sep 11;12(9):2778. doi: 10.3390/nu12092778.
Liver fibrosis is a pathological process with intrahepatic diffused deposition of the excess extracellular matrix, which leads to various chronic liver diseases. Drugs with high efficacy and low toxicity for liver fibrosis are still unavailable. has antioxidant, antivirus, antitumor and anti-inflammation roles, and has been used to treat liver diseases in the population. However, the hepatoprotective effects of spores and the mechanisms behind it have not been investigated. In this study, we evaluate the hepatoprotective effect of spore powder of (SP, 100 mg/kg/day or 200 mg/kg/day) on carbon tetrachloride (CCl)-induced liver fibrosis in mice. SP groups reduced serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities compared with the CCl group. SP also showed a decrease in hydroxyproline (Hyp) content in liver tissues. SP improved cell damage and reduced collagen deposition by H&E, Sirius red and Masson staining. Furthermore, SP down-regulated the mRNA levels of and , and the protein expression of α-smooth muscle actin (α-SMA), collagen I (Col 1), tumor necrosis factor alpha (TNF-α), toll like receptor 4 (TLR4) and nuclear factor-Κb (NF-κB) p65. In summary, SP has an ameliorative effect on hepatic fibrosis, probably by inhibiting the activation of hepatic stellate cells, reducing the synthesis of extracellular matrix.
肝纤维化是一种病理过程，其特征是细胞外基质在肝内弥漫沉积，导致各种慢性肝病。目前尚无高效低毒的抗肝纤维化药物。灵芝具有抗氧化、抗病毒、抗肿瘤和抗炎作用，已被用于人群治疗肝脏疾病。然而，灵芝孢子的保肝作用及其机制尚未得到研究。在这项研究中，我们评估了灵芝孢子粉（SP，每天 100mg/kg 或 200mg/kg）对四氯化碳（CCl）诱导的小鼠肝纤维化的保护作用。SP 组与 CCl 组相比，血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）活性降低。SP 还显示肝组织中羟脯氨酸（Hyp）含量降低。SP 通过 H&E、天狼猩红和 Masson 染色改善细胞损伤和减少胶原沉积。此外，SP 下调 和 的 mRNA 水平，以及α-平滑肌肌动蛋白（α-SMA）、胶原 I（Col 1）、肿瘤坏死因子-α（TNF-α）、Toll 样受体 4（TLR4）和核因子-Κb（NF-κB）p65 的蛋白表达。总之，SP 对肝纤维化具有改善作用，可能通过抑制肝星状细胞的激活，减少细胞外基质的合成。
