Tyurin Alexander A, Suhorukova Alexandra V, Kabardaeva Ksenia V, Goldenkova-Pavlova Irina V
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences (IPP RAS), Moscow 127276, Russia.
Plants (Basel). 2020 Sep 11;9(9):1187. doi: 10.3390/plants9091187.
A large data array on plant gene expression accumulated thanks to comparative omic studies directs the efforts of researchers to the specific or fine effects of the target gene functions and, as a consequence, elaboration of relatively simple and concurrently effective approaches allowing for the insight into the physiological role of gene products. Numerous studies have convincingly demonstrated the efficacy of transient expression strategy for characterization of the plant gene functions. The review goals are (i) to consider the advantages and limitations of different plant systems and methods of transient expression used to find out the role of gene products; (ii) to summarize the current data on the use of the transient expression approaches for the insight into fine mechanisms underlying the gene function; and (iii) to outline the accomplishments in efficient transient expression of plant genes. In general, the review discusses the main and critical steps in each of the methods of transient gene expression in plants; areas of their application; main results obtained using plant objects; their contribution to our knowledge about the fine mechanisms of the plant gene functions underlying plant growth and development; and clarification of the mechanisms regulating complex metabolic pathways.
得益于比较组学研究，积累了大量关于植物基因表达的数据阵列，这引导研究人员关注目标基因功能的特定或精细效应，进而促成相对简单且同时有效的方法，以便深入了解基因产物的生理作用。众多研究令人信服地证明了瞬时表达策略在表征植物基因功能方面的有效性。本综述的目标是：（i）探讨用于探究基因产物作用的不同植物系统和瞬时表达方法的优缺点；（ii）总结关于使用瞬时表达方法深入了解基因功能潜在精细机制的现有数据；（iii）概述植物基因高效瞬时表达方面的成果。总体而言，本综述讨论了植物瞬时基因表达各方法中的主要关键步骤、其应用领域、使用植物对象获得的主要结果、它们对我们了解植物生长发育过程中植物基因功能精细机制的贡献，以及对调节复杂代谢途径机制的阐明。
