Establishment and Validation of an Efficient -Mediated Transient Transformation System for .

, C. Wang & Chang Y. Yang, a desert-adapted shrub, is recognized for its exceptional drought tolerance and plays a vital role in ecosystem maintenance. However, research on has been limited due to the lack of an efficient and reliable genetic transformation method, including gene functional studies. The -mediated transient overexpression assay is a rapid and powerful tool for analyzing gene function in plant vivo. In this study, tissue culture seedlings of were utilized as the recipient materials, and the plant expression vector pCAMBIA1301, containing the reporter gene, was transferred into the seedlings via an -mediated method. To enhance the efficiency of the system, the effects of secondary culture time, concentration, infection time, and co-culture duration on the transient transformation efficiency of were explored. The optimal combination for the instantaneous transformation of tissue culture seedlings mediated by was determined as follows: a secondary culture time of 30 d, a value of OD of 0.8, an infection time of 3 h, and a co-culture duration of 48 h. Subsequently, the effectiveness of the transformation system was validated using the drought response gene . To further confirm the accuracy of the system, -overexpressing was constructed and drought resistance analysis was performed. The results were consistent with the transient overexpression of in tissue culture seedlings, indicating that this system can be effectively employed for studying gene function in . These findings provide essential information for investigating gene function in non-model plants and pave the way for advancements in molecular biology research in .