Ellis J A, Davis W C, Belden E L, Pratt D L
Department of Veterinary Sciences, University of Wyoming, Laramie.
Vet Pathol. 1988 May;25(3):231-6. doi: 10.1177/030098588802500308.
Clinically normal, bovine viral diarrhea virus (BVDV)-seronegative, 7 to 9-month-old steers were inoculated intranasally with NY-1, a noncytopathic strain of BVDV, or exposed intramuscularly to killed BVDV. Indirect immunofluorescence staining with monoclonal antibodies specific for bovine leukocyte subsets followed by flow cytometric analysis was used to monitor subsequent hematologic alterations. Infection with BVDV resulted in a transient leukopenia which was characterized by decreases in the absolute numbers of circulating T lymphocytes, including BoT4+ ("helper") and BoT8+ ("cytotoxic/suppressor") subsets, B lymphocytes, and neutrophils. There was no significant variation in numbers of non-T, non-B ("null") lymphocytes or monocytes. Exposure to inactivated BVDV in a combination vaccine did not cause significant alteration in the circulating numbers of any major leukocyte subset; however, significant variation was seen in the BoT4/BoT8 ratios and in the numbers of cells expressing major histocompatibility complex (MHC) class II antigens.
对临床正常、牛病毒性腹泻病毒（BVDV）血清学阴性、7至9月龄的公牛进行鼻内接种NY-1（一种非细胞病变性BVDV毒株），或肌肉注射灭活的BVDV。使用针对牛白细胞亚群的单克隆抗体进行间接免疫荧光染色，随后进行流式细胞术分析，以监测后续的血液学变化。感染BVDV导致短暂性白细胞减少，其特征是循环T淋巴细胞绝对数量减少，包括BoT4 +（“辅助性”）和BoT8 +（“细胞毒性/抑制性”）亚群、B淋巴细胞和中性粒细胞。非T、非B（“裸”）淋巴细胞或单核细胞数量没有显著变化。在联合疫苗中接触灭活的BVDV不会导致任何主要白细胞亚群的循环数量发生显著改变；然而，在BoT4/BoT8比率以及表达主要组织相容性复合体（MHC）II类抗原的细胞数量上观察到了显著变化。
