Villasante Alejandro, Catalán Natalia, Rojas Rodrigo, Lohrmann Karin B, Romero Jaime
Laboratorio de Biotecnología de los Alimentos, Unidad de Alimentos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, El Líbano 5524, Macul, Santiago 783090, Chile.
Doctorado en Acuicultura, Programa Cooperativo Universidad de Chile, Universidad Católica del Norte, Pontificia Universidad Católica de Valparaíso, Santiago 783090, Chile.
Microorganisms. 2020 Sep 13;8(9):1411. doi: 10.3390/microorganisms8091411.
Withering syndrome (WS), an infectious disease caused by intracellular bacteria Xenohaliotis californiensis, has provoked significant economic losses in abalone aquaculture. The pathogen infects gastroenteric epithelia, including digestive gland, disrupting the digestive system and causing a progressive wilting in abalone. Nonetheless, our knowledge about WS implications in digestive gland microbiota, and its role in diseases progress remains largely unknown. This study aims to determine whether digestive gland-associated microbiota differs between healthy red abalone () and red abalone affected with WS. Using high-throughput sequencing of the V4 region of the 16S rRNA gene, our results revealed differences in microbiota between groups. Bacterial genera, including , , and were significantly more abundant in healthy abalones, whilst Xenohaliotis californiensis and were more abundant in WS-affected abalones. Whilst was the dominant genus in the healthy group, Xenohaliotis californiensis was dominant in the WS group. However, Xenohaliotis californiensis was present in two healthy specimens, and thus the / Xenohaliotis californiensis ratio appears to be more determinant in specimens affected with WS. Further research to elucidate the role of digestive gland microbiota ecology in WS pathogenesis is required.
消瘦综合征（WS）是一种由细胞内细菌加州异鲍菌引起的传染病，已给鲍鱼养殖业造成了重大经济损失。该病原体感染包括消化腺在内的胃肠上皮，破坏消化系统，导致鲍鱼逐渐萎缩。然而，我们对WS对消化腺微生物群的影响及其在疾病进展中的作用仍知之甚少。本研究旨在确定健康红鲍（）和受WS影响的红鲍的消化腺相关微生物群是否存在差异。通过对16S rRNA基因V4区域进行高通量测序，我们的结果揭示了不同组之间微生物群的差异。包括、、和在内的细菌属在健康鲍鱼中明显更为丰富，而加州异鲍菌和在受WS影响的鲍鱼中更为丰富。虽然是健康组中的优势属，但加州异鲍菌在WS组中占主导地位。然而，在两个健康样本中也存在加州异鲍菌，因此/加州异鲍菌的比例似乎在受WS影响的样本中更具决定性。需要进一步研究以阐明消化腺微生物群生态在WS发病机制中的作用。
