School of Women's and Children's Health, University of New South Wales Faculty of Medicine, Sydney, New South Wales, Australia
Microbiome Research Centre, University of New South Wales Faculty of Medicine, Sydney, New South Wales, Australia.
BMJ Open. 2020 Sep 15;10(9):e040189. doi: 10.1136/bmjopen-2020-040189.
Pregnancy induces significant physiological and cardiometabolic changes, and is associated with alterations in the maternal microbiota. Increasing rates of prepregnancy obesity, metabolic abnormalities and reduced physical activity, all impact negatively on the microbiota causing an imbalance between the commensal microorganisms (termed dysbiosis), which may drive complications, such as gestational diabetes or hypertensive disorders. Considerable work is needed to define the inter-relationships between the microbiome, nutrition, physical activity and pregnancy outcomes. The role of the microbiota during pregnancy remains unclear. The aim of the study is to define microbiota signatures longitudinally throughout pregnancy and the first year post birth, and to identify key clinical and environmental variables that shape the female microbiota profile during and following pregnancy.
The Microbiome Understanding in Maternity Study (MUMS) is an Australian prospective longitudinal cohort study involving 100 mother-infant pairs. Women are enrolled in their first trimester and followed longitudinally. Assessment occurs at <13+0, 20+0-24+6 and 32+0-36+6 weeks gestation, birth and 6 weeks, 6 months and 12 months postpartum. At each assessment, self-collected oral, vaginal and faecal samples are collected with an additional postpartum skin swab and breastmilk sample. Each infant will have oral, faecal and skin swab samples collected. Measurements include anthropometrics, body composition, blood pressure, serum hormonal and metabolic parameters and vaginal pH. Dietary intake, physical activity and psychological state will be assessed using validated self-report questionnaires, and pregnancy and infant outcomes recorded. Parametric and non-parametric hypothesis tests will be used to test the association between high-risk and low-risk pregnancies and their outcomes.
The study received the following approval: South Eastern Sydney Local Health District Research Ethics Committee (17/293 (HREC/17/POWH/605). Results will be made available to the participants of MUMS, their families and the funding bodies; in the form of a summary document. Results for the greater maternity care community and other researchers will be disseminated through conferences, local, national and international presentations and peer-reviewed publications.
ACTRN12618000471280 (prospectively registered).
怀孕会引起显著的生理和心血管代谢变化，并与母体微生物组的改变有关。孕前肥胖、代谢异常和体力活动减少的发生率不断上升，这些都对微生物组产生负面影响，导致共生微生物（称为失调）之间的失衡，这可能导致妊娠糖尿病或高血压等并发症。需要大量的工作来定义微生物组、营养、体力活动和妊娠结局之间的相互关系。怀孕期间微生物组的作用尚不清楚。本研究的目的是在整个怀孕期间和产后第一年确定纵向的微生物组特征，并确定塑造怀孕和产后女性微生物组特征的关键临床和环境变量。
母婴微生物组研究（MUMS）是一项澳大利亚前瞻性纵向队列研究，涉及 100 对母婴。在孕早期招募女性，并进行纵向随访。评估发生在<13+0、20+0-24+6 和 32+0-36+6 周妊娠、分娩和产后 6 周、6 个月和 12 个月。在每次评估时，自我采集口腔、阴道和粪便样本，并在产后采集额外的皮肤拭子和母乳样本。每个婴儿将采集口腔、粪便和皮肤拭子样本。测量包括人体测量学、身体成分、血压、血清激素和代谢参数以及阴道 pH 值。通过验证的自我报告问卷评估饮食摄入、体力活动和心理状态，并记录妊娠和婴儿结局。将使用参数和非参数假设检验来检验高危和低危妊娠及其结局之间的关联。
该研究获得了以下批准：东南悉尼地方卫生区研究伦理委员会（17/293（HREC/17/POWH/605）。结果将提供给 MUMS 的参与者、他们的家人和资助机构；以摘要文件的形式。结果将通过会议、当地、国家和国际演讲以及同行评审出版物向更大的产妇保健社区和其他研究人员传播。
ACTRN12618000471280（前瞻性注册）。
