UNSW Microbiome Research Centre, St George and Sutherland Clinical Campuses, UNSW Sydney, Sydney, NSW 2052, Australia.
UNSW Stats Central, Biological Sciences South Building (E26), Level 2 Kensington, UNSW Sydney, Sydney, NSW 2052, Australia.
Int J Environ Res Public Health. 2023 Sep 9;20(18):6736. doi: 10.3390/ijerph20186736.
The microbiome has emerged as a key determinant of human health and reproduction, with recent evidence suggesting a dysbiotic microbiome is implicated in adverse perinatal health outcomes. The existing research has been limited by the sample collection and timing, cohort design, sample design, and lack of data on the preconception microbiome. This prospective, longitudinal cohort study will recruit 2000 Australian women, in order to fully explore the role of the microbiome in the development of adverse perinatal outcomes. Participants are enrolled for a maximum of 7 years, from 1 year preconception, through to 5 years postpartum. Assessment occurs every three months until pregnancy occurs, then during Trimester 1 (5 + 0-12 + 6 weeks gestation), Trimester 2 (20 + 0-24 + 6 weeks gestation), Trimester 3 (32 + 0-36 + 6 weeks gestation), and postpartum at 1 week, 2 months, 6 months, and then annually from 1 to 5 years. At each assessment, maternal participants self-collect oral, skin, vaginal, urine, and stool samples. Oral, skin, urine, and stool samples will be collected from children. Blood samples will be obtained from maternal participants who can access a study collection center. The measurements taken will include anthropometric, blood pressure, heart rate, and serum hormonal and metabolic parameters. Validated self-report questionnaires will be administered to assess diet, physical activity, mental health, and child developmental milestones. Medications, medical, surgical, obstetric history, the impact of COVID-19, living environments, and pregnancy and child health outcomes will be recorded. Multiomic bioinformatic and statistical analyses will assess the association between participants who developed high-risk and low-risk pregnancies, adverse postnatal conditions, and/or childhood disease, and their microbiome for the different sample types.
微生物组已成为人类健康和生殖的关键决定因素，最近的证据表明，失调的微生物组与不良围产期健康结果有关。现有研究受到样本采集和时间、队列设计、样本设计以及缺乏孕前微生物组数据的限制。这项前瞻性、纵向队列研究将招募 2000 名澳大利亚女性，以充分探索微生物组在不良围产期结局发展中的作用。参与者最多可入组 7 年，从孕前 1 年到产后 5 年。评估每 3 个月进行一次，直到怀孕，然后在妊娠 1 期（妊娠 5+0-12+6 周）、妊娠 2 期（妊娠 20+0-24+6 周）、妊娠 3 期（妊娠 32+0-36+6 周）和产后 1 周、2 个月、6 个月进行，然后从 1 年到 5 年每年进行一次。在每次评估时，产妇参与者自行采集口腔、皮肤、阴道、尿液和粪便样本。将从儿童中采集口腔、皮肤、尿液和粪便样本。可以前往研究采集中心的产妇参与者将获得血液样本。所测量的指标包括人体测量学、血压、心率以及血清激素和代谢参数。将使用经过验证的自我报告问卷评估饮食、身体活动、心理健康和儿童发育里程碑。将记录药物、医疗、手术、产科史、COVID-19 的影响、生活环境以及妊娠和儿童健康结局。多组学生物信息学和统计分析将评估不同样本类型中出现高危和低危妊娠、不良产后情况和/或儿童疾病的参与者及其微生物组之间的关联。
