Mori N, Oka K, Kojima M
Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
Am J Clin Pathol. 1988 Aug;90(2):143-8. doi: 10.1093/ajcp/90.2.143.
Lymph nodes from four cases of mantle zone lymphoma (MZL) were studied immunohistochemically. All the cases had serum M proteins. Immunostaining with paraffin-embedded specimens revealed that proliferating cells of neoplastic nodules possessed the same monoclonal immunoglobulins as those in the serum (IgM, kappa in three and IgM, gamma in one). The plasma cells around neoplastic nodules contained the same monoclonal immunoglobulins as those in the nodules in two cases. In the remainder, most plasma cells around the nodules possessed the same immunoglobulins as those in the nodules. The authors consider these plasma cells to be the cause of paraproteinemia. Immunostaining with paraffin and frozen tissue sections revealed that the neoplastic cells (or neoplastic nodules) were positive for sIgD, sIgM, dendritic reticulum cell antigen (DRC-1), and LN-2 and weakly positive for common acute lymphocytic leukemia antigen (CALLA), and LN-1. These results indicate that the neoplastic cells of MZL have both phenotypes of mantle zone lymphocytes (sIgD, sIgM, and LN-2 positive) and of germinal center cells (sIgM, DRC-1, CALLA, and LN-1 positive). MZL is thus considered to be a neoplasma of B-cells differentiating from mantle zone lymphocytes to germinal center cells.
对4例套细胞淋巴瘤（MZL）的淋巴结进行了免疫组织化学研究。所有病例均有血清M蛋白。石蜡包埋标本的免疫染色显示，肿瘤结节的增殖细胞具有与血清中相同的单克隆免疫球蛋白（3例为IgM、κ链，1例为IgM、γ链）。在2例中，肿瘤结节周围的浆细胞含有与结节中相同的单克隆免疫球蛋白。其余病例中，结节周围的大多数浆细胞具有与结节中相同的免疫球蛋白。作者认为这些浆细胞是副蛋白血症的原因。石蜡切片和冰冻组织切片的免疫染色显示，肿瘤细胞（或肿瘤结节）对sIgD、sIgM、树突状网状细胞抗原（DRC-1）和LN-2呈阳性，对常见急性淋巴细胞白血病抗原（CALLA）和LN-1呈弱阳性。这些结果表明，MZL的肿瘤细胞具有套细胞淋巴细胞（sIgD、sIgM和LN-2阳性）和生发中心细胞（sIgM、DRC-1、CALLA和LN-1阳性）两种表型。因此，MZL被认为是一种从套细胞淋巴细胞分化为生发中心细胞的B细胞肿瘤。
