Huang Xin, Chen Zhenhao, Zhao Guanglei, Shi Jingsheng, Huang Gangyong, Chen Feiyan, Wei Yibing, Xia Jun, Chen Jie, Wang Siqun
Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
Exp Ther Med. 2020 Nov;20(5):19. doi: 10.3892/etm.2020.9147. Epub 2020 Aug 27.
Articular cartilage injuries are common orthopedic conditions that severely affect the quality of life of patients. Tissue engineering can facilitate cartilage repair and the key points involve scaffolding and seed cell selection. Pre-experiments found a range of microstructures of bioceramic scaffolds suitable for chondrocyte adhesion and proliferation, and maintaining chondrocyte phenotype. Three-dimensional cultures of bone marrow mesenchymal stem cell (BMSC) scaffolds were implanted into mice. According to the shape of the bioceramic scaffolds and the implantation time , RNA sequencing was performed on the removed scaffolds to explore the molecular mechanism. The bone plate culture can induce differentiation of chondrocytes, making culture different to that produced . Implantation of scaffolds increases the expression of bone-related genes. The ceramic rod-like material was found to be superior to the disc shape, and the bone repair effect was more marked with longer implantation times. Gene Ontology analysis revealed that 'cell chemotaxis', 'negative regulation of ossification' and 'bone development' pathways were involved in recovery. It was further confirmed that BMSCs were suitable as seed cells for cartilage tissue engineering, and that the β-tricalcium phosphate scaffold maybe ideal as cartilage tissue engineering scaffold material. The present research provided new insights into the molecular mechanism of cartilage repair by BMSCs and bioceramic scaffolds. Bioinformatics analysis revealed that AMMECR1L-like protein, tumor necrosis factor-induced protein 2, inhibitor of nuclear factor-B kinase subunit and protein kinase C type and 'negative regulation of ossification' and 'bone development' pathways may be involved in osteoblast maturation and bone regeneration.
关节软骨损伤是常见的骨科病症，严重影响患者的生活质量。组织工程有助于软骨修复，关键在于支架和种子细胞的选择。前期实验发现了一系列适合软骨细胞黏附、增殖并维持软骨细胞表型的生物陶瓷支架微观结构。将骨髓间充质干细胞（BMSC）支架的三维培养物植入小鼠体内。根据生物陶瓷支架的形状和植入时间，对取出的支架进行RNA测序以探索分子机制。骨板培养可诱导软骨细胞分化，使培养物与所产生的不同。支架植入增加了骨相关基因的表达。发现陶瓷棒状材料优于圆盘形状，且植入时间越长，骨修复效果越明显。基因本体分析表明，“细胞趋化性”“骨化的负调控”和“骨发育”途径参与了恢复过程。进一步证实BMSC适合作为软骨组织工程的种子细胞，且β-磷酸三钙支架可能是理想的软骨组织工程支架材料。本研究为BMSC和生物陶瓷支架修复软骨的分子机制提供了新见解。生物信息学分析表明，类AMMECR1L蛋白、肿瘤坏死因子诱导蛋白2、核因子-κB激酶亚基抑制剂和蛋白激酶C类型以及“骨化的负调控”和“骨发育”途径可能参与成骨细胞成熟和骨再生。
