Siqueira L, Passador F R, Costa M M, Lobo A O, Sousa E
Laboratory of Bioceramics, Institute of Science and Technology, UNIFESP, São José dos Campos, SP, Brazil.
Laboratory of Polymer Processing, Institute of Science and Technology, UNIFESP, São José dos Campos, SP, Brazil.
Mater Sci Eng C Mater Biol Appl. 2015;52:135-43. doi: 10.1016/j.msec.2015.03.055. Epub 2015 Mar 26.
Electrospinning is a simple and low-cost way to fabricate fibers. Among the various polymers used in electrospinning process, the poly (lactic acid) (PLA) stands out due to its excellent biodegradability and biocompatibility. Calcium phosphate ceramics has been recognized as an attractive biomaterial because their chemical composition is similar to the mineral component of the hard tissue in the body. Furthermore, they are bioactive and osteoinductive and some are even quite biodegradable. The beta-tricalcium phosphate (β-TCP) particles were synthesized by solid state reaction. Different contents of β-TCP particles were incorporated in polymer matrices to form fibers of PLA/β-TCP composites by electrospinning aiming a possible application as a scaffold for tissue engineering. The fibers were characterized by scanning electron microscopy (SEM), infrared (FTIR), differential scanning calorimetry (DSC) and thermal gravimetric analysis (TGA). The average diameter of the fibers varied in the range of 260-519.6 ± 50 nm. The presence of β-TCP particles promoted changes on thermal properties of the fibers. The composite with 8 wt-% of β-TCP showed a low degree of crystallinity and can be used for application in tissue engineering. The cell viability was analyzed by reduction of the methyl tetrazolium salt by the pyruvate dehydrogenase enzymatic complex present in the matrix of mitochondria (MTT test). All PLA fiber groups, with different contents of β-TCP, showed cytocompatibility ability with non-cytotoxicity effect and bioactive properties using SBF assay.
静电纺丝是一种简单且低成本的制备纤维的方法。在静电纺丝过程中使用的各种聚合物中，聚乳酸（PLA）因其优异的生物降解性和生物相容性而脱颖而出。磷酸钙陶瓷已被公认为一种有吸引力的生物材料，因为它们的化学成分与人体硬组织的矿物质成分相似。此外，它们具有生物活性和骨诱导性，有些甚至具有相当好的生物降解性。通过固态反应合成了β-磷酸三钙（β-TCP）颗粒。将不同含量的β-TCP颗粒掺入聚合物基质中，通过静电纺丝形成PLA/β-TCP复合材料纤维，旨在作为组织工程支架进行潜在应用。通过扫描电子显微镜（SEM）、红外光谱（FTIR）、差示扫描量热法（DSC）和热重分析（TGA）对纤维进行了表征。纤维的平均直径在260 - 519.6 ± 50 nm范围内变化。β-TCP颗粒的存在促进了纤维热性能的变化。含有8 wt-%β-TCP的复合材料显示出低结晶度，可用于组织工程应用。通过线粒体基质中存在的丙酮酸脱氢酶复合物还原甲基四氮唑盐来分析细胞活力（MTT试验）。使用SBF试验，所有含有不同含量β-TCP的PLA纤维组均显示出细胞相容性，具有无细胞毒性作用和生物活性。
