Wu Junlu, Wang Xuetao, Shang Anquan, Vella Giovanna, Sun Zujun, Ji Ping, Yang Dianyu, Wan Aiming, Yao Yiwen, Li Dong
Department of Clinical Laboratory, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
Department of Radiology, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China.
Oncol Lett. 2020 Nov;20(5):128. doi: 10.3892/ol.2020.11989. Epub 2020 Aug 19.
Placenta-specific 8 (PLAC8) is closely associated with the proliferation, apoptosis and autophagy of several tumor cells. However, the expression and function of PLAC8 in oral squamous cell carcinoma (OSCC) remain unknown. Therefore, the present study investigated the function and mechanism of PLAC8 in OSCC. Reverse transcription-quantitative PCR and western blot analyses were performed to quantify the expression of PLAC8 in OSCC cell lines. The function of PLAC8 in OSCC was investigated via transfection, the Transwell and Cell Counting Kit-8 assays, immunofluorescence staining and western blotting. The results demonstrated that PLAC8 exspression was downregulated in OSCC cell lines. PLAC8 inhibited the cell proliferation in OSCC. In addition, PLAC8 restrained invasion and epithelial-mesenchymal transition of OSCC cells. Furthermore, β-catenin helped to repress PLAC8 expression by regulating the Wnt/β-catenin and PI3K/Akt/GSK3β signaling pathways in OSCC cells. Collectively, the results of the present study suggest that PLAC8 acts as a tumor suppressor in OSCC by downregulating β-catenin.
胎盘特异性8（PLAC8）与多种肿瘤细胞的增殖、凋亡和自噬密切相关。然而，PLAC8在口腔鳞状细胞癌（OSCC）中的表达和功能仍不清楚。因此，本研究探讨了PLAC8在OSCC中的功能和机制。采用逆转录定量PCR和蛋白质印迹分析来量化PLAC8在OSCC细胞系中的表达。通过转染、Transwell和细胞计数试剂盒-8检测、免疫荧光染色和蛋白质印迹研究了PLAC8在OSCC中的功能。结果表明，PLAC8在OSCC细胞系中的表达下调。PLAC8抑制OSCC中的细胞增殖。此外，PLAC8抑制OSCC细胞的侵袭和上皮-间质转化。此外，β-连环蛋白通过调节OSCC细胞中的Wnt/β-连环蛋白和PI3K/Akt/GSK3β信号通路来抑制PLAC8的表达。总的来说，本研究结果表明，PLAC8通过下调β-连环蛋白在OSCC中发挥肿瘤抑制作用。
