Criminal Technology Detachment of Haikou Public Security Bureau, Haikou, China.
School of Basic Medical Sciences & Forensic Medicine, Hangzhou Medical College, Hangzhou, China.
Int J Legal Med. 2020 Nov;134(6):2075-2077. doi: 10.1007/s00414-020-02424-8. Epub 2020 Sep 15.
Insertion/null polymorphisms (INNULs) can be used as an alternative marker of STRs to detect the highly degraded samples in forensic cases. In this study, we evaluated the genetic data of 20 INNUL markers in the Innotyper® 21 Human DNA Analysis Kit (InnoGenomics) for Hainan Li population, including allele frequencies and forensic parameters. No significant deviation from Hardy-Weinberg equilibrium and linkage disequilibrium was found in all loci after Bonferroni correction. The combined power of discrimination (CPD) was 0.99999891, the combined power of exclusion for duo paternity testing (CPEduo) was 0.75274389, and the combined power of exclusion for trio paternity testing (CPEtrio) was 0.94766143. These data would be useful for the application of the kit in practice and the research of the kit used in molecular anthropology studies.
插入/缺失多态性(INNULs)可作为 STR 的替代标记,用于检测法医案件中高度降解的样本。在这项研究中,我们评估了 Innotyper® 21 人类 DNA 分析试剂盒(InnoGenomics)中 20 个 INNUL 标记在海南黎族人群中的遗传数据,包括等位基因频率和法医参数。经过 Bonferroni 校正后,所有位点均未发现显著偏离 Hardy-Weinberg 平衡和连锁不平衡。复合鉴别力(CPD)为 0.99999891,双人亲子鉴定的复合排除力(CPEduo)为 0.75274389,三人亲子鉴定的复合排除力(CPEtrio)为 0.94766143。这些数据将有助于试剂盒在实践中的应用以及试剂盒在分子人类学研究中的应用。