Analytical Chemistry Group, van't Hoff Institute for Molecular Sciences (HIMS), Faculty of Science, University of Amsterdam, Amsterdam, The Netherlands.
Centre for Analytical Sciences Amsterdam, Amsterdam, The Netherlands.
J Sep Sci. 2021 Jan;44(1):63-87. doi: 10.1002/jssc.202000768. Epub 2020 Oct 4.
Accurate quantification of polymer distributions is one of the main challenges in polymer analysis by liquid chromatography. The response of contemporary detectors is typically influenced by compositional features such as molecular weight, chain composition, end groups, and branching. This renders the accurate quantification of complex polymers of which there are no standards available, extremely challenging. Moreover, any (programmed) change in mobile-phase composition may further limit the applicability of detection techniques. Current methods often rely on refractive index detection, which is not accurate when dealing with complex samples as the refractive-index increment is often unknown. We review current and emerging detection methods in liquid chromatography with the aim of identifying detectors, which can be applied to the quantitative analysis of complex polymers.
准确量化聚合物分布是液相色谱法分析聚合物的主要挑战之一。现代检测器的响应通常受到组成特征的影响，如分子量、链组成、端基和支化。这使得对没有标准品的复杂聚合物的准确定量变得极具挑战性。此外，任何（编程的）流动相组成的变化都可能进一步限制检测技术的适用性。目前的方法通常依赖于折射率检测，但是当处理复杂样品时，折射率增量通常是未知的，因此这种方法并不准确。我们综述了液相色谱中的现有和新兴检测方法，旨在确定可以应用于复杂聚合物定量分析的检测器。
