National Forestry and Grassland Bureau Woody Spice (East China) Engineering Technology Research Center, the Institute of Plant Natural Products and Forest Products Chemical Engineering, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China.
Molecules. 2023 Feb 24;28(5):2132. doi: 10.3390/molecules28052132.
At present, the technology used for the extraction and purification of saponins generally has the problems of high cost and low purity, and the quantitative detection of saponins also has the problems of low sensitivity and easy interference from impurities. To solve these problems, this paper aimed to use liquid chromatography for the quantitative detection of saponins, and to adjust and optimize the related conditions. In our study, the average recovery of saponins obtained was 100.42%. The RSD of precision test was 0.41%. The RSD of the repeatability test was 0.22%. The detection limit of the liquid chromatography was 0.06 mg/L, and the quantification limit was 0.2 mg/L. In order to improve the yield and purity, the saponins were extracted from Abel. seed meal by methanol extraction. Then, the extracted saponins were extracted with an ammonium sulfate/propanol aqueous two-phase system. We optimized the purification process of formaldehyde extraction and aqueous two-phase extraction. Under the optimal purification process, the purity of saponins extracted by methanol was 36.15%, and the yield was 25.24%. The purity of saponins obtained by aqueous two-phase extraction was 83.72%. Thus, this study can provide a reference standard for rapid and efficient detection and analysis of saponins for industrial extraction and purification.
目前，用于提取和纯化皂苷的技术通常存在成本高、纯度低的问题，皂苷的定量检测也存在灵敏度低、易受杂质干扰的问题。为了解决这些问题，本文旨在使用液相色谱法对皂苷进行定量检测，并对相关条件进行调整和优化。在我们的研究中，皂苷的平均回收率为 100.42%。精密度试验的 RSD 为 0.41%。重复性试验的 RSD 为 0.22%。液相色谱的检测限为 0.06mg/L，定量限为 0.2mg/L。为了提高收率和纯度，采用甲醇从 Abel 种子粕中提取皂苷。然后，用硫酸铵/丙醇双水相体系提取提取的皂苷。我们优化了甲醛提取和双水相萃取的纯化工艺。在最佳的纯化工艺下，甲醇提取的皂苷的纯度为 36.15%，收率为 25.24%。双水相提取的皂苷的纯度为 83.72%。因此，本研究可为工业提取和纯化中皂苷的快速、高效检测和分析提供参考标准。
