Quantitative Analysis of Seed Saponins and Aqueous Two-Phase Extraction and Separation.

At present, the technology used for the extraction and purification of saponins generally has the problems of high cost and low purity, and the quantitative detection of saponins also has the problems of low sensitivity and easy interference from impurities. To solve these problems, this paper aimed to use liquid chromatography for the quantitative detection of saponins, and to adjust and optimize the related conditions. In our study, the average recovery of saponins obtained was 100.42%. The RSD of precision test was 0.41%. The RSD of the repeatability test was 0.22%. The detection limit of the liquid chromatography was 0.06 mg/L, and the quantification limit was 0.2 mg/L. In order to improve the yield and purity, the saponins were extracted from Abel. seed meal by methanol extraction. Then, the extracted saponins were extracted with an ammonium sulfate/propanol aqueous two-phase system. We optimized the purification process of formaldehyde extraction and aqueous two-phase extraction. Under the optimal purification process, the purity of saponins extracted by methanol was 36.15%, and the yield was 25.24%. The purity of saponins obtained by aqueous two-phase extraction was 83.72%. Thus, this study can provide a reference standard for rapid and efficient detection and analysis of saponins for industrial extraction and purification.