Department of Biology and Food Science, Shangqiu Normal University , Shangqiu, Henan, China.
State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, School of Life Sciences, Department of Biology, Henan University , Kaifeng, Henan, China.
Plant Signal Behav. 2020 Dec 1;15(12):1816321. doi: 10.1080/15592324.2020.1816321. Epub 2020 Sep 16.
The role of Heat Shock Transcription Factor 6 ( & ) in response to abiotic stresses such as ABA, drought, salinity, drought, and osmotic stress is individually well established. Unfortunately, the functional redundancy between the and as well as the consequences of simultaneous editing of both in response to aforementioned stresses remains elusive. Therefore, this study was designed with the aim of addressing whether there is any functional redundancy between and as well as to decipher their role in abiotic stresses tolerance in , by using the CRISPR-Cas9. We have generated the single () as well as double mutants ( and ) of and with higher frequencies of deletion, insertion, and substitution. The phenotypic characterization of generated double and single mutants under abiotic stresses such as ABA, mannitol, and NaCl identified double mutants more tolerant to subjected abiotic stresses than those of their single mutants. It warrants mentioning that we have identified that and also involved in other major ABA responses, including ABA-inhibited seed germination, stomatal movement, and water loss. In addition to the above, the simultaneous editing of and lead to a reduced ROS accumulation, accompanied by increased expression of much abiotic stress and ABA-responsive genes, including involved in regulation of ROS level. In conclusion, these results suggest that and may offer abiotic stress tolerance by regulating the ROS homeostasis in plants.
热休克转录因子 6（ & ）在应对非生物胁迫方面的作用，如 ABA、干旱、盐度、干旱和渗透胁迫，已经得到了很好的证实。不幸的是， 与 之间的功能冗余，以及同时编辑这两者以应对上述胁迫的后果仍然难以捉摸。因此，本研究旨在探讨 与 之间是否存在功能冗余，并利用 CRISPR-Cas9 技术阐明它们在拟南芥非生物胁迫耐受性中的作用。我们已经成功地生成了 与 的单突变体（ ）和双突变体（ 和 ），并且这些突变体的删除、插入和取代频率更高。对生成的双突变体和单突变体在 ABA、甘露醇和 NaCl 等非生物胁迫下的表型特征进行了分析，结果表明双突变体比其单突变体更能耐受所施加的非生物胁迫。值得一提的是，我们还发现 与 还参与了其他主要的 ABA 反应，包括 ABA 抑制种子萌发、气孔运动和水分损失。除此之外，同时编辑 与 会导致 ROS 积累减少，同时增加许多非生物胁迫和 ABA 响应基因的表达，包括参与 ROS 水平调节的基因。总之，这些结果表明， 与 可能通过调节植物中的 ROS 稳态来提供非生物胁迫耐受性。
