Guo Qing, Wei Ran, Xu Min, Yao Wenjing, Jiang Jiahui, Ma Xujun, Qu Guanzheng, Jiang Tingbo
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
School of Architecture and Civil Engineer, Heilongjiang University of Science and Technology, Harbin, China.
Front Plant Sci. 2023 Apr 26;14:1160102. doi: 10.3389/fpls.2023.1160102. eCollection 2023.
Heat shock transcription factor (HSF) is an important TF that performs a dominant role in plant growth, development, and stress response network. In this study, we identified a total of 30 HSF members from poplar, which are unevenly distributed on 17 chromosomes. The poplar HSF family can be divided into three subfamilies, and the members of the same subfamily share relatively conserved domains and motifs. HSF family members are acidic and hydrophilic proteins that are located in the nucleus and mainly carry out gene expansion through segmental replication. In addition, they have rich collinearity across plant species. Based on RNA-Seq analysis, we explored the expression pattern of under salt stress. Subsequently, we cloned the significantly upregulated gene and transformed it into × . Under salt stress, the transgenic poplar overexpressing had a better growth state and higher reactive oxygen scavenging ability. A yeast one-hybrid experiment indicated PtHSF21 could improve salt tolerance by specifically binding to the anti-stress cis-acting element HSE. This study comprehensively profiled the fundamental information of poplar HSF family members and their responses to salt stress and specifically verified the biological function of , which provides clues for understanding the molecular mechanism of poplar HSF members in response to salt stress.
热激转录因子（HSF）是一种重要的转录因子，在植物生长、发育和胁迫响应网络中发挥着主导作用。在本研究中，我们从杨树中总共鉴定出30个HSF成员，它们不均匀地分布在17条染色体上。杨树HSF家族可分为三个亚家族，同一亚家族的成员具有相对保守的结构域和基序。HSF家族成员是酸性和亲水性蛋白质，定位于细胞核，主要通过片段复制进行基因扩张。此外，它们在不同植物物种间具有丰富的共线性。基于RNA测序分析，我们探究了盐胁迫下的表达模式。随后，我们克隆了显著上调的基因并将其转化到×中。在盐胁迫下，过表达的转基因杨树具有更好的生长状态和更高的活性氧清除能力。酵母单杂交实验表明，PtHSF21可以通过特异性结合抗逆顺式作用元件HSE来提高耐盐性。本研究全面描述了杨树HSF家族成员的基本信息及其对盐胁迫的响应，并具体验证了的生物学功能，为理解杨树HSF成员响应盐胁迫的分子机制提供了线索。
