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牛的胞浆内单精子注射。

Intracytoplasmic Sperm Injection in Cattle.

机构信息

Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N4N1, Canada.

出版信息

Genes (Basel). 2021 Jan 29;12(2):198. doi: 10.3390/genes12020198.

DOI:10.3390/genes12020198
PMID:33572865
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7911995/
Abstract

Intracytoplasmic sperm injection (ICSI) involves the microinjection of sperm into a matured oocyte. Although this reproductive technology is successfully used in humans and many animal species, the efficiency of this procedure is low in the bovine species mainly due to failed oocyte activation following sperm microinjection. This review discusses various reasons for the low efficiency of ICSI in cattle, potential solutions, and future directions for research in this area, emphasizing the contributions of testis-specific isoforms of Na/K-ATPase (ATP1A4) and phospholipase C zeta (PLC ζ). Improving the efficiency of bovine ICSI would benefit the cattle breeding industries by effectively utilizing semen from elite sires at their earliest possible age.

摘要

胞质内精子注射 (ICSI) 涉及将精子微注射到成熟的卵母细胞中。尽管这项生殖技术在人类和许多动物物种中得到了成功应用，但在牛种中，该程序的效率很低，主要是由于精子微注射后卵母细胞激活失败。本综述讨论了牛种 ICSI 效率低下的各种原因、潜在的解决方案以及该领域的未来研究方向，强调了睾丸特异性 Na/K-ATPase（ATP1A4）和磷脂酶 C ζ（PLC ζ）同工型的贡献。提高牛 ICSI 的效率将通过在尽可能早的年龄有效利用优秀种公牛的精液，使牛的繁殖产业受益。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baab/7911995/52d73d7067e3/genes-12-00198-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baab/7911995/967ac0a2b3e2/genes-12-00198-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baab/7911995/7c79c52afc85/genes-12-00198-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baab/7911995/52d73d7067e3/genes-12-00198-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baab/7911995/967ac0a2b3e2/genes-12-00198-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baab/7911995/7c79c52afc85/genes-12-00198-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baab/7911995/52d73d7067e3/genes-12-00198-g003.jpg

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本文引用的文献

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Na/K-ATPase and Regulation of Sperm Function.钠钾ATP酶与精子功能的调节
Anim Reprod. 2018 Aug 3;15(Suppl 1):711-720. doi: 10.21451/1984-3143-AR2018-0024. eCollection 2018 Jul-Sep.
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Gamevar.f90: a software package for calculating individual gametic diversity.Gamevar.f90：一个用于计算个体配子多样性的软件包。
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Testis-Specific Isoform of Na/K-ATPase (ATP1A4) Interactome in Raft and Non-Raft Membrane Fractions from Capacitated Bovine Sperm.
磷脂酶Cζ1（PLCZ1）：在牛和马的生育力评估及体外胚胎生产中的功能与潜力
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SPERM FACTORS AND EGG ACTIVATION: ICSI and the discovery of the sperm factor and PLCZ1.精子因素与卵子激活：ICSI 技术与精子因子和 PLCZ1 的发现。
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Mysteries and unsolved problems of mammalian fertilization and related topics.哺乳动物受精的奥秘和未解之谜及相关课题。
Biol Reprod. 2022 Apr 26;106(4):644-675. doi: 10.1093/biolre/ioac037.
顶体反应中牛精子质膜筏和非筏膜蛋白相互作用组分析：睾丸特异性 Na+/K+-ATP 酶（ATP1A4）同工型
Int J Mol Sci. 2019 Jun 28;20(13):3159. doi: 10.3390/ijms20133159.
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Detrimental effects of oxidative stress in bovine oocytes during intracytoplasmic sperm injection (ICSI).氧化应激对牛卵母细胞在胞质内单精子注射（ICSI）过程中的有害影响。
Theriogenology. 2019 Jul 15;133:71-78. doi: 10.1016/j.theriogenology.2019.04.012. Epub 2019 Apr 11.
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