Division of Diagnostic Imaging, Department of Radiology, Japan Labour Health and Safety Organization, Tokyo Rosai Hospital, Ohta-ku, Tokyo, Japan.
Methods Mol Biol. 2023;2519:41-51. doi: 10.1007/978-1-0716-2433-3_5.
Chromosome analysis is one of most fundamental techniques for cytogenetic studies. Chromosomes are conventionally prepared from mitotic cells arrested by colcemid block protocol. Premature chromosome condensation (PCC) technique is an alternative to obtain chromosomes. It was more than half century ago that the first observation of PCC phenomena reported. Since then, cell-fusion-mediated PCC method has been developed and introduced in many fields of chromosome analysis. More than quarter century ago, novel PCC technique using chemical drug has been developed. Afterwards, this simple and efficient drug-induced PCC technique becomes a standard protocol for preparing chromosomes. Thus, it seems to be the good time to introduce PCC technique protocol for the artisans in the field of cytogenetic studies.
染色体分析是细胞遗传学研究中最基本的技术之一。染色体通常是通过秋水仙素阻断法从有丝分裂细胞中制备的。早熟染色体凝聚（PCC）技术是获得染色体的另一种方法。早在半个多世纪前，就首次报道了 PCC 现象的观察结果。从那时起，细胞融合介导的 PCC 方法已经在染色体分析的许多领域得到了发展和应用。二十多年前，人们开发了一种使用化学药物的新型 PCC 技术。此后，这种简单高效的药物诱导 PCC 技术成为制备染色体的标准方案。因此，现在似乎是向细胞遗传学研究领域的专家介绍 PCC 技术方案的好时机。
