Singh B, Ho M, Looareesuwan S, Mathai E, Warrell D A, Hommel M
Wolfson Tropical Immunology Unit, Liverpool School of Tropical Medicine, UK.
Clin Exp Immunol. 1988 Apr;72(1):145-50.
The effect of sera on the cytoadherence in vitro of Plasmodium falciparum-infected erythrocytes to melanoma cells was examined. Sera from 19 healthy individuals living in endemic malarious areas in Thailand and 24 patients with P. falciparum malaria were tested against four local P. falciparum isolates. Out of 57 sera examined, 12 (21%) showed significant inhibition (greater than 50%) of cytoadherence for at least one isolate. Anti-malarial IgG antibody titres were determined for all 57 sera and although 11 of the 12 inhibitory sera had relatively high titres, 36 out of 47 sera with similarly high titres showed no significant inhibitory activity. Convalescent sera were no more effective than corresponding acute sera in inhibiting the cytoadherence of erythrocytes infected with any of the four heterologous isolates examined. Sera which significantly inhibited cytoadherence were also capable of reversing cytoadherence, and pooled plasma, from healthy individuals living in malarious areas, was effective in significantly reversing the in vitro cytoadherence of all the five parasite isolates examined. The results confirm the antibody mediated strain-specific nature of the inhibition of cytoadherence and stress the difficulty in selecting immune sera potentially useful for the immunotherapy of cerebral malaria patients in Thailand.
研究了血清对恶性疟原虫感染的红细胞在体外与黑色素瘤细胞细胞粘附的影响。检测了来自泰国疟疾流行地区的19名健康个体和24名恶性疟患者的血清，针对4种当地恶性疟原虫分离株进行测试。在检测的57份血清中，12份（21%）对至少一种分离株表现出显著的细胞粘附抑制作用（大于50%）。测定了所有57份血清的抗疟IgG抗体滴度，虽然12份抑制性血清中有11份具有相对较高的滴度，但47份具有同样高滴度的血清中有36份未表现出显著的抑制活性。恢复期血清在抑制感染4种异源分离株中任何一种的红细胞的细胞粘附方面并不比相应的急性期血清更有效。显著抑制细胞粘附的血清也能够逆转细胞粘附，并且来自疟疾流行地区健康个体的混合血浆能够有效显著逆转所检测的所有5种寄生虫分离株的体外细胞粘附。结果证实了抗体介导的细胞粘附抑制的菌株特异性性质，并强调了在泰国选择可能对脑型疟患者免疫治疗有用的免疫血清的困难。
