Wang Feixiang, Zhou Panting, Li Ke, Mamtilahun Muyassar, Tang Yaohui, Du Guohao, Deng Biao, Xie Honglan, Yang Guoyuan, Xiao Tiqiao
Shanghai Synchrotron Radiation Facility/Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201204, People's Republic of China.
Neuroscience and Neuroengineering Research Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China.
IUCrJ. 2020 Jul 11;7(Pt 5):793-802. doi: 10.1107/S2052252520008234. eCollection 2020 Sep 1.
Early stages of diseases, including stroke, hypertension, angiogenesis of tumours, spinal cord injuries, ., are closely associated with the lesions of microvasculature. Rodent models of human vascular diseases are extensively used for the preclinical investigation of the disease evolution and therapy with synchrotron radiation. Therefore, non-invasive and X-ray imaging with high sensitivity and clarity is desperately needed to visualize the microvessels in live-animal models. Contrast agent is essential for the X-ray imaging of vessels and angiomatous tissue. Because of the non-rigid motion of adjacent tissues, the short circulation time and the intermittent flow of contrast agents in vessels, it is a great challenge for the traditional X-ray imaging methods to achieve well defined images of microvessels . In this article, move contrast X-ray imaging (MCXI) based on high-brightness synchrotron radiation is developed to overcome the intrinsic defects in conventional methods. Experiments with live rodents demonstrate the practicability of the MCXI method for sensitive and intact imaging of microvessels .
包括中风、高血压、肿瘤血管生成、脊髓损伤等在内的疾病早期，都与微血管病变密切相关。人类血管疾病的啮齿动物模型被广泛用于同步辐射对疾病进展和治疗的临床前研究。因此，迫切需要高灵敏度和清晰度的非侵入性X射线成像来观察活体动物模型中的微血管。造影剂对于血管和血管瘤组织的X射线成像是必不可少的。由于相邻组织的非刚性运动、造影剂在血管中的短循环时间和间歇性流动，传统的X射线成像方法要获得清晰的微血管图像是一项巨大的挑战。在本文中，基于高亮度同步辐射的移动造影X射线成像（MCXI）被开发出来以克服传统方法的固有缺陷。对活体啮齿动物的实验证明了MCXI方法对微血管进行灵敏且完整成像的实用性。
