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使用像素时间平均法对活体小鼠脑微血管进行实时 X 射线成像。

Real-time X-ray imaging of mouse cerebral microvessels in vivo using a pixel temporal averaging method.

机构信息

Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204, People's Republic of China.

Shanghai Synchrotron Radiation Facility/Zhang Jiang Lab, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201800, People's Republic of China.

出版信息

J Synchrotron Radiat. 2022 Jan 1;29(Pt 1):239-246. doi: 10.1107/S1600577521012522.

DOI:10.1107/S1600577521012522
PMID:34985441
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8733992/
Abstract

Rodents are used extensively as animal models for the preclinical investigation of microvascular-related diseases. However, motion artifacts in currently available imaging methods preclude real-time observation of microvessels in vivo. In this paper, a pixel temporal averaging (PTA) method that enables real-time imaging of microvessels in the mouse brain in vivo is described. Experiments using live mice demonstrated that PTA efficiently eliminated motion artifacts and random noise, resulting in significant improvements in contrast-to-noise ratio. The time needed for image reconstruction using PTA with a normal computer was 250 ms, highlighting the capability of the PTA method for real-time angiography. In addition, experiments with less than one-quarter of photon flux in conventional angiography verified that motion artifacts and random noise were suppressed and microvessels were successfully identified using PTA, whereas conventional temporal subtraction and averaging methods were ineffective. Experiments performed with an X-ray tube verified that the PTA method could also be successfully applied to microvessel imaging of the mouse brain using a laboratory X-ray source. In conclusion, the proposed PTA method may facilitate the real-time investigation of cerebral microvascular-related diseases using small animal models.

摘要

啮齿动物被广泛用作微血管相关疾病临床前研究的动物模型。然而，目前可用的成像方法中的运动伪影妨碍了在体实时观察微血管。在本文中，描述了一种像素时间平均（PTA）方法，该方法能够实时成像活体小鼠大脑中的微血管。使用活鼠的实验表明，PTA 有效地消除了运动伪影和随机噪声，从而显著提高了对比噪声比。使用普通计算机进行 PTA 图像重建所需的时间为 250 毫秒，突出了 PTA 方法进行实时血管造影的能力。此外，在传统血管造影中光子通量减少四分之一的实验验证了 PTA 可以抑制运动伪影和随机噪声，并成功识别微血管，而传统的时间减影和平均方法则无效。使用 X 射线管进行的实验验证了 PTA 方法也可以成功应用于使用实验室 X 射线源的小鼠大脑微血管成像。总之，所提出的 PTA 方法可能有助于使用小动物模型实时研究与脑微血管相关的疾病。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/5d8799c7f874/s-29-00239-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/7db8a9367fbe/s-29-00239-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/1c3b4ec71435/s-29-00239-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/3d47caabdbd4/s-29-00239-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/611158018b8d/s-29-00239-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/74693d7c3c62/s-29-00239-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/038247f464f6/s-29-00239-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/4ea33fea5f9f/s-29-00239-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/5d8799c7f874/s-29-00239-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/7db8a9367fbe/s-29-00239-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/1c3b4ec71435/s-29-00239-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/3d47caabdbd4/s-29-00239-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/611158018b8d/s-29-00239-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/74693d7c3c62/s-29-00239-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/038247f464f6/s-29-00239-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/4ea33fea5f9f/s-29-00239-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb6/8733992/5d8799c7f874/s-29-00239-fig8.jpg

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