上皮-间质转化过程中 E-钙黏蛋白的分子张力显微镜
Molecular Tension Microscopy of E-Cadherin During Epithelial-Mesenchymal Transition.
机构信息
Institut Jacques Monod, Université de Paris, CNRS, Paris, France.
Faculty of Basic and Biomedical Sciences, Université de Paris, Paris, France.
出版信息
Methods Mol Biol. 2021;2179:289-299. doi: 10.1007/978-1-0716-0779-4_22.
Molecular Tension Microscopy has been increasingly used in the last years to investigate mechanical forces acting in cells at the molecular scale. Here, we describe a protocol to image the tension of the junctional protein E-cadherin in cultured epithelial cells undergoing Epithelial-Mesenchymal Transition (EMT). We report how to prepare cells and induce EMT, and how to acquire, analyze, and quantitatively interpret FRET data.
分子张力显微镜在过去几年中被越来越多地用于研究细胞在分子尺度上的机械力。在这里，我们描述了一种在经历上皮-间充质转化（EMT）的培养上皮细胞中成像连接蛋白 E-钙黏蛋白张力的方案。我们报告了如何准备细胞并诱导 EMT，以及如何获取、分析和定量解释 FRET 数据。
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