环状 RNA circ-NCOR2 通过海绵吸附 miR-516a-5p 来上调转移相关蛋白 2 的表达，从而促进甲状腺乳头状癌的进展。

Circular RNA circ-NCOR2 accelerates papillary thyroid cancer progression by sponging miR-516a-5p to upregulate metastasis-associated protein 2 expression.

机构信息

Department of Nuclear Medicine, the Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.

Department of Nuclear Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

出版信息

J Int Med Res. 2020 Sep;48(9):300060520934659. doi: 10.1177/0300060520934659.

OBJECTIVE

Papillary thyroid cancer (PTC) is one of the most prevalent endocrine malignancies and the fifth most common cancer in women. Circular RNAs (circRNAs) have been shown to play vital functions in cancers, but few studies have focused on the functions and mechanism of dysregulated circRNAs in PTC.

METHODS

Quantitative reverse transcription PCR was used to measure circ-NCOR2 levels in PTC tissues and cell lines. The functions of circ-NCOR2 in PTC were examined by analysis using the cell counting kit-8, clone forming, flow cytometry, and Transwell experiments. Bioinformatic analysis and dual luciferase reporter gene testing were used to identify the mechanisms of circ-NCOR2.

RESULTS

Circ-NCOR2 overexpression was observed in PTC tissues and cells. Silenced or overexpressed expression of circ-NCOR2 strikingly attenuated or facilitated, respectively, the growth, migration, and invasion of PTC cells. Mechanistically, miR-615a-5p was identified as the target of circ-NCOR2. Moreover, circ-NCOR2 enhanced the expression of metastasis-associated protein 2 (MTA2) by sponging miR-615a-5p, thereby facilitating PTC cell progression.

CONCLUSIONS

Taken together, our findings reveal a novel circ-NCOR2/miR-615a-5p/MTA2 axis in PTC, which could become a potential therapeutic target for this disease.

目的

甲状腺乳头状癌（PTC）是最常见的内分泌恶性肿瘤之一，也是女性中第五大常见癌症。环状 RNA（circRNAs）已被证明在癌症中发挥重要作用，但很少有研究关注失调 circRNAs 在 PTC 中的功能和机制。

方法

采用定量逆转录 PCR 检测 PTC 组织和细胞系中 circ-NCOR2 的水平。通过细胞计数试剂盒-8、克隆形成、流式细胞术和 Transwell 实验分析 circ-NCOR2 在 PTC 中的功能。生物信息学分析和双荧光素酶报告基因检测用于鉴定 circ-NCOR2 的机制。

结果

circ-NCOR2 在 PTC 组织和细胞中表达上调。沉默或过表达 circ-NCOR2 可显著抑制或促进 PTC 细胞的生长、迁移和侵袭。机制上，miR-615a-5p 被鉴定为 circ-NCOR2 的靶基因。此外，circ-NCOR2 通过海绵吸附 miR-615a-5p 增强了转移相关蛋白 2（MTA2）的表达，从而促进了 PTC 细胞的进展。