Protein adsorption and bioactivity of functionalized electrospun membranes for bone regeneration.

OBJECTIVES

To evaluate the adsorption of bone related proteins and bioactivity of experimental functionalized (carboxylated or aminated) polymeric membranes for bone regeneration.

METHODS

Polymethylmethacrylate-based membranes functionalized with carboxyl or amino radicals were tested. Membranes were zinc loaded and the adsorption isotherms of zinc were studied. Human plasma proteins, bovine serum albumin, fibrinogen and fibronectin adsorption were measured with a spectrophotometer applying an acid determination protocol. Biomimetic calcium phosphate precipitation on polymeric membranes was also assessed after simulated body fluid immersion. Scanning electron microscopy and elemental analysis by means of an energy dispersive system were used for mineral deposits identification. A commercially available polytetrafluoroethylene membrane was used as control.

RESULTS

Both experimental membranes produced higher protein adsorption than the commercial control that does not adsorb proteins. Carboxylated membranes adsorbed significantly more albumin than the aminated ones, the opposite occurred with fibrinogen. With plasma and fibronectin proteins both type of membranes performed similarly. Only carboxylated membranes were bioactive and precipitated calcium and phosphate on their surfaces.

CONCLUSIONS

The polymethylmethacrylate zinc-loaded membranes functionalized with carboxyl groups performed as high adsorbable membranes for bone regeneration related proteins. They also served as templates for mineralization of hydroxyapatite.

CLINICAL SIGNIFICANCE

Protein adsorption is the initial reaction after the implantation of a biomaterial into the body and will influence subsequent cell function. The adsorption of bone related proteins together with the observed biomimetic calcium deposition on the experimental carboxylated membranes could be associated with their ability to promote bone regeneration.