Faculty of Dentistry, Biomaterials. University of Granada. Campus Cartuja sn. E-18071, Granada, Spain.
Faculty of Dentistry, Biomaterials. University of Granada. Campus Cartuja sn. E-18071, Granada, Spain.
J Dent. 2020 Jul;98:103359. doi: 10.1016/j.jdent.2020.103359. Epub 2020 May 5.
To assess the surface characteristics and composition that may enhance osteoblasts viability on novel electrospun composite membranes (organic polymer/silicon dioxide nanoparticles).
Membranes are composed by a novel polymer blend, the mixture of two hydrophilic copolymers 2-hydroxyethylmethacrylate-co-methylmethacrylate and 2-hydroxyethylacrylate-co-methylacrylate, and they are doped with silicon dioxide nanoparticles. Then the membranes were functionalized with zinc or doxycycline. The membranes were morphologically characterized by atomic force and scanning electron microscopy (FESEM), and mechanically probed using a nanoindenter. Biomimetic calcium phosphate precipitation on polymeric tissues was assessed. Cell viability tests were performed using human osteosarcoma cells. Cells morphology was also studied by FESEM. Data were analyzed by ANOVA, Student-Newman-Keuls and Student t tests (p < 0.05).
Silica doping of membranes enhanced bioactivity and increased mechanical properties. Membranes morphology and mechanical properties were similar to those of trabecular bone. Zinc and doxycycline doping did not exert changes but it increased novel membranes bioactivity. Membranes were found to permit osteoblasts proliferation. Silica-doping favored cells proliferation and spreading. As soon as 24 h after the seeding, cells in silica-doped membranes were firmly attached to experimental tissues trough filopodia, connected to each other. The cells produced collagen and minerals onto the surfaces.
Silica nanoparticles enhanced surface properties and osteoblasts viability on electrospun membranes.
The ability of silica-doped matrices to promote precipitation of calcium phosphate, together with their mechanical properties, observed non-toxicity, stimulating effect on osteoblasts and its surface chemistry allowing covalent binding of proteins, offer a potential strategy for bone regeneration applications.
评估可能增强新型电纺复合膜（有机聚合物/二氧化硅纳米粒子）上成骨细胞活力的表面特性和组成。
膜由一种新型聚合物共混物组成，该共混物由两种亲水性共聚物 2-羟乙基甲基丙烯酸酯-共-甲基丙烯酸甲酯和 2-羟乙基丙烯酸酯-共-甲基丙烯酸酯组成，并掺杂有二氧化硅纳米粒子。然后，将膜用锌或强力霉素功能化。通过原子力显微镜和扫描电子显微镜（FESEM）对膜进行形态学表征，并使用纳米压痕仪进行力学探测。评估聚合物组织上仿生钙磷酸盐的沉淀。使用人骨肉瘤细胞进行细胞活力测试。还通过 FESEM 研究了细胞形态。通过方差分析、Student-Newman-Keuls 和 Student t 检验（p < 0.05）对数据进行分析。
膜中二氧化硅的掺杂增强了生物活性并提高了机械性能。膜的形态和机械性能与小梁骨相似。锌和强力霉素的掺杂没有改变，但增加了新型膜的生物活性。发现膜允许成骨细胞增殖。二氧化硅掺杂有利于细胞增殖和扩展。在接种后 24 小时内，通过丝状伪足，细胞牢固地附着在实验组织上，并相互连接。细胞在表面上产生胶原蛋白和矿物质。
二氧化硅纳米粒子增强了电纺膜的表面特性和成骨细胞活力。
观察到掺杂二氧化硅的基质具有促进钙磷酸盐沉淀的能力、其机械性能、非毒性、对成骨细胞的刺激作用以及其表面化学允许蛋白质的共价结合，为骨再生应用提供了一种潜在策略。
