Graham K A, Buick R N
Ontario Cancer Institute, Toronto, Canada.
J Cell Physiol. 1988 Jul;136(1):63-71. doi: 10.1002/jcp.1041360108.
Addition of sodium butyrate (NaB) to 6 cultured human breast carcinoma cell lines results in a dose and time-dependent growth inhibition. Kinetic evidence, related to the growth of a minority cell population which decreases in size with time of exposure, is presented to indicate that the NaB effect is reversible. In those cell lines that express the estrogen receptor (ER), growth inhibition is accompanied by a more differentiated phenotype, which is characterized by increased accumulation of lipid and milk-fat globule membrane glycoproteins. The potential for differentiation is not blocked by tamoxifen, indicating that the relationship to ER expression is likely secondary to the association of ER expression with a particular stage of secretory cell differentiation that is susceptible to NaB induction. Of the 3 lines shown to respond in this way (MCF-7, ZR-75-1, and MDA-134), ZR-75-1 is an extreme example that may serve as a model for studies of gene expression during human mammary epithelial cell differentiation.
向6种培养的人乳腺癌细胞系中添加丁酸钠（NaB）会导致剂量和时间依赖性的生长抑制。文中提供了与少数细胞群体生长相关的动力学证据，该群体随着暴露时间的延长而变小，表明NaB的作用是可逆的。在那些表达雌激素受体（ER）的细胞系中，生长抑制伴随着更分化的表型，其特征是脂质和乳脂肪球膜糖蛋白的积累增加。他莫昔芬不会阻断分化的可能性，这表明与ER表达的关系可能继发于ER表达与易受NaB诱导的分泌细胞分化特定阶段的关联。在显示出这种反应的3种细胞系（MCF-7、ZR-75-1和MDA-134）中，ZR-75-1是一个极端例子，可作为研究人乳腺上皮细胞分化过程中基因表达的模型。
