van der Fels Christa A M, Palthe Selma, Buikema Henk, van den Heuvel Marius C, Leliveld Annemarie, de Jong Igle Jan
Department of Urology, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands.
Department of Pathology, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands.
Diagnostics (Basel). 2020 Sep 15;10(9):694. doi: 10.3390/diagnostics10090694.
Imaging modalities using tumor-directed monoclonal antibodies may be of value to improve the pre- and intraoperative detection and resection of lymph node (LN) metastatic disease in penile squamous cell carcinoma (PSCC). We investigated the expression of prostate-specific membrane antigen (PSMA), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR) and epithelial cell adhesion molecule (EpCAM) to analyze their potency for diagnostic applications. Antigen expression was determined in primary tumors and LNs with and without metastases of 22 patients with PSCC. The total immunostaining score (TIS, 0-12) was determined as the product of a proportion score (PS, 0-4) and an intensity score (IS, 0-3). EGFR and VEGF expression were high in primary tumor (median TIS 8) and LN metastases (median TIS 6 and 8, respectively). No EGFR expression was seen in LNs without metastases. However, LNs without metastases did show VEGF expression (median TIS 6). No EpCAM or PSMA expression was seen in PSCC. This study shows that VEGF and EGFR expression is moderate to high in LN metastases of PSCC. Both VEGF and EGFR warrant further clinical evaluation to determine their value as a target for pre- and intraoperative imaging modalities in the detection of LN metastases in PSCC.
使用肿瘤导向性单克隆抗体的成像方式可能有助于改善阴茎鳞状细胞癌（PSCC）中淋巴结（LN）转移疾病的术前和术中检测及切除。我们研究了前列腺特异性膜抗原（PSMA）、血管内皮生长因子（VEGF）、表皮生长因子受体（EGFR）和上皮细胞粘附分子（EpCAM）的表达，以分析它们在诊断应用中的效能。在22例PSCC患者的原发性肿瘤和有或无转移的LN中测定抗原表达。总免疫染色评分（TIS，0 - 12）被确定为比例评分（PS，0 - 4）和强度评分（IS，0 - 3）的乘积。EGFR和VEGF在原发性肿瘤（中位TIS 8）和LN转移灶（中位TIS分别为6和8）中表达较高。在无转移的LN中未见EGFR表达。然而，无转移的LN确实显示出VEGF表达（中位TIS 6）。在PSCC中未见EpCAM或PSMA表达。本研究表明，VEGF和EGFR在PSCC的LN转移灶中表达为中度至高度。VEGF和EGFR都需要进一步的临床评估，以确定它们作为PSCC中LN转移检测的术前和术中成像方式靶点的价值。
