Queen Mary School, Nanchang University, Nanchang, Jiangxi, 330027, China.
The Department of Hepatopancreatobillary Surgery, Xuzhou City Cancer Hospital, Xuzhou, Jiangsu 221005, China.
Curr Pharm Biotechnol. 2021;22(8):1106-1113. doi: 10.2174/1389201021999200917144704.
Pancreatic Ductal Adenocarcinoma (PDAC) is the most common and deadly cancer. Surgical resection is the only possible cure for pancreatic cancer but often has a poor prognosis, and the role of adjuvant therapy is urgently explored.
MicroRNAs (miRNAs) play a very important role in tumorigenesis by regulating the target genes. In this study, we identified miR-320b lower-expressed in human pancreatic cancer tissues but relatively higher-expressed in the adjacent non-tumor tissues.
Consistently, the expression of miR-320b in different pancreatic cancer cell lines was significantly lower than the normal pancreatic cells. In order to identify the effects of miR-320b on cell growth, we overexpressed miR-320b in PANC-1 and FG pancreatic cancer cell lines, CCK8 and BrdU incorporation assay results showed that miR-320b inhibited cell proliferation.
We next predicted miR-320b targeted FOXM1 (Forkhead box protein M1) and identified the negative relationship between miR-320b and FOXM1. We also demonstrated that elevated miR- 320b expression inhibited tumor growth in vivo.
All of these results showed that miR-320b suppressed pancreatic cancer cell proliferation by targeting FOXM1, which might provide a new diagnostic marker for pancreatic cancer.
胰腺导管腺癌（PDAC）是最常见和最致命的癌症。手术切除是胰腺癌唯一可能治愈的方法，但预后往往较差，迫切需要探索辅助治疗的作用。
微小 RNA（miRNAs）通过调节靶基因在肿瘤发生中起着非常重要的作用。在本研究中，我们发现 miR-320b 在人胰腺癌细胞组织中表达较低，但在相邻非肿瘤组织中表达相对较高。
一致地，不同胰腺癌细胞系中 miR-320b 的表达明显低于正常胰腺细胞。为了确定 miR-320b 对细胞生长的影响，我们在 PANC-1 和 FG 胰腺癌细胞系中转染了 miR-320b，CCK8 和 BrdU 掺入实验结果表明 miR-320b 抑制细胞增殖。
接下来，我们预测了 miR-320b 靶向 FOXM1（叉头框蛋白 M1）并确定了 miR-320b 和 FOXM1 之间的负相关关系。我们还证明了升高的 miR-320b 表达抑制了体内肿瘤的生长。
所有这些结果表明，miR-320b 通过靶向 FOXM1 抑制胰腺癌细胞增殖，这可能为胰腺癌提供一个新的诊断标志物。
