Wang Ziyu, Li Jiao, Xu Jing, Wan Tingqiu, Ya Yunjin, Li Xi, Wang Xi, Jin Yan
Department of Hepatobiliary and Pancreatic Surgery, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, No. 157 Jinbi Road, Xishan District, Kunming, 650100, Yunnan, China.
Discov Oncol. 2025 May 7;16(1):680. doi: 10.1007/s12672-025-02472-9.
Cholangiocarcinoma, a cancer of the biliary duct, is frequently diagnosed in advanced stages with poor prognosis. Understanding the molecular mechanisms driving its progression is crucial for developing effective treatment approaches. This research delves into the role of LINC00313 in regulating the aggressiveness of cholangiocarcinoma cells.
Clinical samples were collected to examine the expression pattern of LINC00313, miR-320b, and MITF using RT-qPCR and western blot analysis. Functional experiments, including cell proliferation, migration, and invasion assays, as well as apoptosis detection, were performed to assess the malignant properties of cholangiocarcinoma cells. The tumorigenic potential of cholangiocarcinoma cells was further investigated in a xenograft mouse model.
Elevated levels of LINC00313 were detected in both cholangiocarcinoma tumors and cell lines. Knocking down LINC00313 reduced the aggressiveness of cholangiocarcinoma cells and hindered their ability to form tumors in vivo. It was discovered that miR-320b is a target of LINC00313, exhibiting an expression pattern and functional role that contrasts with LINC00313. Moreover, the LINC00313/miR-320b axis was found to regulate the expression of MITF, thereby influencing the aggressiveness of cholangiocarcinoma cells.
LINC00313/miR-320b/MITF axis is implicated in the malignant progression of cholangiocarcinoma, indicating the potential of targeting LINC00313 as a strategy for cholangiocarcinoma clinical management.
胆管癌是一种胆管癌症，通常在晚期被诊断出来，预后较差。了解驱动其进展的分子机制对于开发有效的治疗方法至关重要。本研究深入探讨了LINC00313在调节胆管癌细胞侵袭性中的作用。
收集临床样本，采用逆转录定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹分析来检测LINC00313、miR-320b和小眼相关转录因子（MITF）的表达模式。进行了包括细胞增殖、迁移和侵袭试验以及细胞凋亡检测在内的功能实验，以评估胆管癌细胞的恶性特性。在异种移植小鼠模型中进一步研究胆管癌细胞的致瘤潜力。
在胆管癌肿瘤和细胞系中均检测到LINC00313水平升高。敲低LINC00313可降低胆管癌细胞的侵袭性，并阻碍其在体内形成肿瘤的能力。发现miR-320b是LINC00313的一个靶点，其表达模式和功能作用与LINC00313相反。此外，发现LINC00313/miR-320b轴调节MITF的表达，从而影响胆管癌细胞的侵袭性。
LINC00313/miR-320b/MITF轴与胆管癌的恶性进展有关，表明靶向LINC00313作为胆管癌临床治疗策略的潜力。
