Kassapidou Maria, Hjalmarsson Lars, Johansson Carina B, Hammarström Johansson Petra, Morisbak Else, Wennerberg Ann, Franke Stenport Victoria
Department of Prosthodontics/Dental Materials Science, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Box 450, SE-405 30 Göteborg, Sweden; Department of Prosthetic Dentistry, Institute for Postgraduate Dental Education, Box 1030, SE-551 11 Jönköping, Sweden.
Department of Prosthodontics/Dental Materials Science, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Box 450, SE-405 30 Göteborg, Sweden; Specialist Dental Clinic, Folktandvården Sörmland AB, The Mälar Hospital, SE-631 88 Eskilstuna, Sweden; Centre for Clinical Research Sörmland, Uppsala University, SE-631 88 Eskilstuna, Sweden.
Dent Mater. 2020 Nov;36(11):e352-e363. doi: 10.1016/j.dental.2020.08.012. Epub 2020 Sep 14.
To investigate the metal ion release, surface roughness and cytoxicity for Co-Cr alloys produced by different manufacturing techniques before and after heat treatment. In addition, to evaluate if the combination of materials affects the ion release.
Five Co-Cr alloys were included, based on four manufacturing techniques. Commercially pure titanium, CpTi grade 4 and a titanium alloy were included for comparison. The ion release tests involved both Inductive Coupled Plasma Optical Emission Spectrometry and Inductive Coupled Plasma Mass Spectrometry analyses. The surface analysis was conducted with optical interferometry. Cells were indirectly exposed to the materials and cell viability was evaluated with the MTT (3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide) method.
All alloys showed a decrease of the total ion release when CpTi grade 4 was present. The total ion release decreased over time for all specimens and the highest ion release was observed from the cast and milled Co-Cr alloy in acidic conditions. The cast and laser-melted Co-Cr alloy and the titanium alloy became rougher after heat treatment. All materials were within the limits of cell viability according to standards.
The ion release from Co-Cr alloys is influenced by the combination of materials, pH and time. Surface roughness is influenced by heat treatment. Furthermore, both ion release and surface roughness are influenced by the manufacturing technique and the alloy type. The clinical implication needs to be further investigated.
研究不同制造工艺生产的钴铬合金在热处理前后的金属离子释放、表面粗糙度和细胞毒性。此外，评估材料组合是否会影响离子释放。
基于四种制造工艺纳入了五种钴铬合金。纳入商业纯钛、4级纯钛（CpTi grade 4）和一种钛合金进行比较。离子释放测试包括电感耦合等离子体发射光谱法和电感耦合等离子体质谱分析法。表面分析采用光学干涉测量法。使细胞间接暴露于材料，并采用MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）法评估细胞活力。
当存在4级纯钛时，所有合金的总离子释放均减少。所有试样的总离子释放随时间减少，在酸性条件下，铸造和铣削的钴铬合金的离子释放最高。铸造和激光熔化的钴铬合金以及钛合金在热处理后变得更粗糙。根据标准，所有材料的细胞活力均在限度内。
钴铬合金的离子释放受材料组合、pH值和时间的影响。表面粗糙度受热处理影响。此外，离子释放和表面粗糙度均受制造工艺和合金类型的影响。其临床意义需要进一步研究。
