Perveen Shagufta, Saeed Muhammad, Parveen Abida, Javed Muhammad Tariq, Zafar Sara, Iqbal Naeem
Department of Botany, Government College University, Faisalabad, 38000 Pakistan.
Physiol Mol Biol Plants. 2020 Sep;26(9):1787-1797. doi: 10.1007/s12298-020-00861-8. Epub 2020 Aug 14.
A pot experiment was conducted to examine the effect of foliar application of various levels of ZnSO on wheat ( L.) under cadmium (Cd) stress. Seeds of two wheat varieties i.e., Ujala-2016 and Anaj-2017 were sown in sand filled plastic pots. Cadmium (CdCl) stress i.e., 0 and 0.5 mM CdCl was applied in full strength Hoagland's nutrient solution after 4 weeks of seed germination. Foliar spray of varying ZnSO levels i.e., 0, 2, 4, 6 and 8 mM was applied after 2 weeks of CdCl stress induction (of 6 week old plants). After 3 weeks of foliar treatment leaf samples of 9 week old wheat plants were collected for the determination of changes in various growth and physiobiochemical attributes. Results obtained showed that cadmium stress (0.5 mM CdCl) significantly decreased shoot and root fresh and dry weights, shoot and root lengths, yield attributes, chlorophyll contents and total phenolics, while increased hydrogen peroxide (HO), total soluble proteins, free proline, glycinebetaine (GB) contents, and activities of antioxidant enzymes i.e., catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POD). Foliar application of varying ZnSO levels significantly increased various growth attributes, chlorophyll contents, HO, free proline, GB and activities of antioxidant enzymes i.e., CAT, POD and APX, while decreased relative water contents and total phenolics under Cd stress or non stress conditions. Furthermore, both wheat varieties showed differential response under Cd stress and towards foliar application of ZnSO e.g., wheat var. Ujala-2016 was higher in shoot dry weight, root length, root fresh and dry weights, total leaf area per plant, 100 grains weight, number of tillers per plant, chlorophyll , hydrogen peroxide (HO), activities of APX, POD, glycinebetaine and leaf free proline contents, while var. Anaj-2017 exhibited high shoot fresh weight, grain yield per plant, no. of grains per plant, chlorophyll contents, chlorophyll ratio, total phenolics, MDA and total soluble protein contents under cadmium stress or non stress conditions.
进行了一项盆栽试验，以研究在镉（Cd）胁迫下，叶面喷施不同水平硫酸锌（ZnSO）对小麦（品种名未完整给出）的影响。将两个小麦品种即Ujala - 2016和Anaj - 2017的种子播种在装满沙子的塑料盆中。在种子萌发4周后，在完全强度的霍格兰营养液中施加镉（CdCl）胁迫，即0和0.5 mM CdCl。在诱导CdCl胁迫2周后（对6周龄植株），叶面喷施不同水平的硫酸锌，即0、2、4、6和8 mM。叶面处理3周后，采集9周龄小麦植株的叶片样本，用于测定各种生长和生理生化指标的变化。结果表明，镉胁迫（0.5 mM CdCl）显著降低了地上部和根部的鲜重和干重、地上部和根部长度、产量指标、叶绿素含量和总酚含量，同时增加了过氧化氢（H₂O₂）、总可溶性蛋白、游离脯氨酸、甘氨酸甜菜碱（GB）含量以及抗氧化酶即过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）和过氧化物酶（POD）的活性。在镉胁迫或非胁迫条件下，叶面喷施不同水平的硫酸锌显著增加了各种生长指标、叶绿素含量、H₂O₂、游离脯氨酸、GB以及抗氧化酶即CAT、POD和APX的活性，同时降低了相对含水量和总酚含量。此外，两个小麦品种在镉胁迫下以及对叶面喷施硫酸锌的反应存在差异，例如，Ujala - 2016品种在地上部干重、根长、根鲜重和干重、单株总叶面积、百粒重、单株分蘖数、叶绿素、过氧化氢（H₂O₂）、APX、POD、甘氨酸甜菜碱和叶片游离脯氨酸含量方面较高，而Anaj - 2017品种在镉胁迫或非胁迫条件下，地上部鲜重、单株籽粒产量、单株粒数、叶绿素含量、叶绿素比值、总酚、丙二醛和总可溶性蛋白含量较高。
