Böcher Sarah, Meyer Hendrik L, Dafni Evdokia, Conrads Georg
Department of Operative Dentistry, Periodontology and Preventive Dentistry, Rheinisch-Westfälische Technische Hochschule (RWTH) University Hospital, Pauwelsstrasse 30, 52074 Aachen, Germany.
Division of Oral Microbiology and Immunology, Department of Operative Dentistry, Periodontology and Preventive Dentistry, Rheinisch-Westfälische Technische Hochschule (RWTH) University Hospital, Pauwelsstrasse 30, 52074 Aachen, Germany.
Antibiotics (Basel). 2023 Sep 19;12(9):1458. doi: 10.3390/antibiotics12091458.
() is a key pathogen involved in periodontal diseases. The aim of this study was to investigate the prevalence and phylogenetic origin of the lipoprotein-gene in its most virulent variant, (co-transcribed with as locus ), in different strains collected worldwide. A total of 138 strains were analyzed for the presence of by pooled analysis and subsequently individual PCRs. Sequencing a core fragment of of the individual strains made it possible to carry out a phylogenetic classification using sequence alignment. In total, 22 of the 138 strains tested positive for , corresponding to a prevalence of 16%. The fragment investigated was highly conserved, with variations in the base sequence detected in only three strains (OMI 1072, OMI 1081, and OMI 1074). In two strains, namely OMI 1072 (original name: I-433) and OMI 1081 (original name: I-372), which originate from monkeys, two amino-acid alterations were apparent. Since has also been found in animal strains, it may be concluded that was transferred from animals to humans and that this originally virulent variant was weakened by mutations over time so that new, less virulent, adapted commensal versions of (, , ), with as the host, evolved.
（）是参与牙周疾病的关键病原体。本研究的目的是调查脂蛋白基因在其最具毒性变体（与作为基因座的共转录）中的流行情况和系统发育起源，该变体存在于全球收集的不同菌株中。通过合并分析和随后的个体聚合酶链反应（PCR），对总共138株菌株进行了的检测。对个体菌株的核心片段进行测序，使得利用序列比对进行系统发育分类成为可能。在总共138株检测的菌株中，有22株对呈阳性，患病率为16%。所研究的片段高度保守，仅在三株菌株（OMI 1072、OMI 1081和OMI 1074）中检测到碱基序列的变异。在两株源自猴子的菌株中，即OMI 1072（原名：I-433）和OMI 1081（原名：I-372），有两个氨基酸改变明显。由于在动物菌株中也发现了，因此可以得出结论，是从动物转移到人类的，并且随着时间的推移，这种最初具有毒性的变体因突变而减弱，从而进化出了以作为宿主的新的、毒性较小的、适应共生的版本（、、）。
