Hočevar Luka, Kovač Jernej, Podkrajšek Katarina Trebušak, Battelino Saba, Pavlič Alenka
Department of Paediatric and Preventive Dentistry, Faculty of Medicine, University of Ljubljana, Hrvatski trg 6, Ljubljana 1000, Slovenia.
University Children's Hospital, University Medical Centre Ljubljana, Bohoričeva 20, Ljubljana, Slovenia.
Data Brief. 2020 Aug 25;32:106224. doi: 10.1016/j.dib.2020.106224. eCollection 2020 Oct.
All children, who were born in 2004 and had undergone surgical treatment for recurrent acute tonsillitis and/or acute otitis media at the ear, nose and throat clinic (ENT) between 2004 and 2010, were called on dental examination and blood sampling. Out of 441 invitees, 113 children and their parents/legal guardians agreed to participate. The following data from this group of subjects are presented: the presence of clinical signs of molar-incisor hypomineralisation (MIH), the distribution of human leukocyte antigen (HLA) alleles DQ2 and DQ8 and eight single nucleotide polymorphisms (SNPs) located in amelogenesis-related genes (rs3796704 in the gene, rs546778141 in the gene, rs2106416 in the gene, rs7660807 and rs35286445 in the gene, rs4870723 in the gene, rs2245803 in the gene, and rs3828054 in the gene). Data on clinical signs of MIH were collected in accordance with the recommendation and on the proposed MIH clinical data recording sheet [1], and with appropriate preliminary training and calibration. Data on HLA DQ2 and DQ8 haplotypes and on SNPs of amelogenesis-related genes were obtained using DNA isolated from blood samples taken from subjects. The HLA DQ2 and DQ8 alleles were determined using the EliGene® Coeliac RT Kits (90,048-RT; Elisabeth Pharmacon spol. s.r.o., Brno-Židenice, Czech Republic) on a 7500 Fast RT-PCR System (Applied Biosystems, Waltham, MA, USA). The distributions of SNPs in the amelogenesis-related genes were determined using high resolution melting (HRM) using the Type-IT HRM Master Mix (Qiagen), TaqMan genotyping assays (ID: C__25766207_10; Thermo Fisher Scientific, Waltham, MA, USA) with the TaqMan Universal Master Mix II, or Sanger sequencing using sequencing master mix BigDye® Terminator v3.1 (Applied Biosystems) and ABI 3500 Genetic Analyser (Applied Biosystems). L. Hočevar, J. Kovač, K. Trebušak Podkrajšek, S. Battelino, A. Pavlič, 2020. The possible influence of genetic aetiological factors on molar-incisor hypomineralisation, Arch. Oral. Biol. 118, 104848. https://doi.org/10.1016/j.archoralbio.2020.104848.
所有在2004年出生、于2004年至2010年间在耳鼻喉科诊所（ENT）因复发性急性扁桃体炎和/或急性中耳炎接受过手术治疗的儿童，均被邀请进行牙科检查和血液采样。在441名被邀请者中，113名儿童及其父母/法定监护人同意参与。呈现了该组受试者的以下数据：磨牙-切牙矿化不全（MIH）的临床体征、人类白细胞抗原（HLA）等位基因DQ2和DQ8的分布以及位于釉质形成相关基因中的八个单核苷酸多态性（SNP）（基因中的rs3796704、基因中的rs546778141、基因中的rs2106416、基因中的rs7660807和rs35286445、基因中的rs4870723、基因中的rs2245803以及基因中的rs3828054）。MIH临床体征的数据是根据建议并在拟议的MIH临床数据记录表[1]上收集的，并经过适当的初步培训和校准。HLA DQ2和DQ8单倍型以及釉质形成相关基因SNP的数据是使用从受试者采集的血液样本中分离的DNA获得的。使用EliGene®乳糜泻RT试剂盒（90,048-RT；Elisabeth Pharmacon spol. s.r.o.，布尔诺-日德尼采，捷克共和国）在7500 Fast RT-PCR系统（美国应用生物系统公司，沃尔瑟姆，马萨诸塞州）上测定HLA DQ2和DQ8等位基因。使用Type-IT HRM Master Mix（Qiagen）通过高分辨率熔解（HRM）、使用TaqMan通用PCR预混液II的TaqMan基因分型分析（ID：C__25766207_10；美国赛默飞世尔科技公司，沃尔瑟姆，马萨诸塞州）或使用测序预混液BigDye® Terminator v3.1（应用生物系统公司）和ABI 3500遗传分析仪（应用生物系统公司）进行Sanger测序来测定釉质形成相关基因中SNP的分布。L. Hočevar、J. Kovač、K. Trebušak Podkrajšek、S. Battelino、A. Pavlič，2020年。遗传病因学因素对磨牙-切牙矿化不全的可能影响，《口腔生物学杂志》，第118卷，104848页。https://doi.org/10.1016/j.archoralbio.2020.104848 。
