Moreddu Rosalia, Elsherif Mohamed, Adams Hadie, Moschou Despina, Cordeiro Maria F, Wolffsohn James S, Vigolo Daniele, Butt Haider, Cooper Jonathan M, Yetisen Ali K
Department of Chemical Engineering, Imperial College London, London SW7 2AZ, UK.
Lab Chip. 2020 Nov 7;20(21):3970-3979. doi: 10.1039/d0lc00438c. Epub 2020 Sep 18.
In this article, using the integration of paper microfluidics within laser-inscribed commercial contact lenses, we demonstrate the multiplexed detection of clinically relevant analytes including hydrogen ions, proteins, glucose, nitrites and l-ascorbic acid, all sampled directly from model tears. In vitro measurements involved the optimization of colorimetric assays, with readouts collected, stored and analyzed using a bespoke Tears Diagnostics smartphone application prototype. We demonstrate the potential of the device to perform discrete measurements either for medical diagnosis or disease screening in the clinic or at the point-of-care (PoC), with future applications including monitoring of ocular infections, uveitis, diabetes, keratopathies and assessing oxidative stress.
在本文中，我们通过将纸质微流体集成到激光刻写的商用隐形眼镜中，展示了对包括氢离子、蛋白质、葡萄糖、亚硝酸盐和l - 抗坏血酸在内的临床相关分析物的多重检测，所有这些分析物均直接从模拟泪液中采样。体外测量涉及比色测定法的优化，使用定制的泪液诊断智能手机应用程序原型收集、存储和分析读数。我们展示了该设备在临床或护理点（PoC）进行医学诊断或疾病筛查的离散测量的潜力，其未来应用包括监测眼部感染、葡萄膜炎、糖尿病、角膜病以及评估氧化应激。
