Masamsetti V Pragathi, Tam Patrick P L
Embryology Unit, Children's Medical Research Institute, University of Sydney, Westmead, NSW, Australia.
Faculty of Medicine and Health, School of Medical Sciences, University of Sydney, Westmead, NSW, Australia.
Methods Mol Biol. 2022;2490:39-45. doi: 10.1007/978-1-0716-2281-0_4.
Mouse embryo studies are pivotal for the understanding of early development. Analysis of the spatial and temporal changes of protein expression during development of a mouse embryo allows us to identify the genetic basis of errors of development in animal disease models. Immunofluorescence is a powerful technique to study the localization and variation in expression pattern of specific proteins in cells, tissues, and organs. Detecting the antigens with their specific antibodies labeled with fluorescent probes allows visualization of proteins at the cellular level. Here, we provide the optimized protocol of immunostaining whole mouse embryos at embryonic stages E7.5 to E11.5.
小鼠胚胎研究对于理解早期发育至关重要。分析小鼠胚胎发育过程中蛋白质表达的时空变化，有助于我们确定动物疾病模型中发育缺陷的遗传基础。免疫荧光是一种强大的技术，可用于研究特定蛋白质在细胞、组织和器官中的定位及表达模式变化。用荧光探针标记的特异性抗体检测抗原，能够在细胞水平可视化蛋白质。在此，我们提供了对胚胎期E7.5至E11.5的全小鼠胚胎进行免疫染色的优化方案。
