Université de Reims Champagne Ardenne, EA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), Reims, France.
Biomater Sci. 2020 Oct 21;8(20):5763-5773. doi: 10.1039/d0bm00962h. Epub 2020 Sep 18.
A multifunctional material system that kills bacteria and drives bone healing is urgently sought to improve bone prosthesis. Herein, the osteoinductive coating made of calcium phosphate/chitosan/hyaluronic acid, named Hybrid, was proposed as an antibacterial substrate for stromal cell adhesion. This Hybrid coating possesses a contact-killing effect reducing by 90% the viability of Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Pseudomonas aeruginosa (P. aeruginosa) strains after 48 h of contact. In addition to the production of immunomodulatory mediators, Wharton's jelly (WJ-SCs), dental pulp (DPSCs) and bone marrow (BM-MSCs) derived stromal cells were able to release antibacterial and antibiofilm agents effective against S. aureus and P. aeruginosa strains, respectively. Studying the effect of the Hybrid coating on the internalization of S. aureus by the stromal cells, in acute-mimicking bone infection, highlighted an increase in the bacteria internalization by DPSCs and BM-MSCs when cultured on the Hybrid coating versus uncoated glass. Despite the internalization, Hybrid coating showed a beneficial effect by reducing the pathogenicity of the internalized bacteria. The formation of biofilm was reduced by at least 50% in comparison to internalized bacteria by stromal cells on uncoated glass. This work opens the route for the development of innovative antibacterial coatings by taking into account the internalization of bacteria by stromal cells.
一种多功能材料系统，既能杀菌又能促进骨骼愈合，迫切需要改善骨假体。在此，提出了一种由磷酸钙/壳聚糖/透明质酸组成的具有成骨诱导作用的涂层（命名为 Hybrid），作为基质细胞黏附的抗菌基底。这种 Hybrid 涂层具有接触杀灭效果，经过 48 小时接触后，革兰氏阳性金黄色葡萄球菌（S. aureus）和革兰氏阴性铜绿假单胞菌（P. aeruginosa）的存活率降低了 90%。除了产生免疫调节介质外，牙髓基质细胞（DPSCs）和成骨基质细胞（BM-MSCs）和牙髓基质细胞（DPSCs）还能够释放针对金黄色葡萄球菌和铜绿假单胞菌的抗菌和抗生物膜剂。研究 Hybrid 涂层对基质细胞内化金黄色葡萄球菌的影响，在模拟急性骨感染的情况下，与未涂层玻璃相比，当 DPSCs 和 BM-MSCs 在 Hybrid 涂层上培养时，细菌内化明显增加。尽管发生了内化，但 Hybrid 涂层通过降低内化细菌的致病性表现出有益的效果。与未涂层玻璃上基质细胞内化的细菌相比，生物膜的形成至少减少了 50%。这项工作为考虑基质细胞内化细菌的创新抗菌涂层的开发开辟了道路。
